Effects of Different Combinations of Silage Bacteria, Cellulolytic Enzymes and Carbon Sources on Fermentation Quality of Rice Straw Silage

OUNGYANG Jialiang, QI Ruxin, CHEN Yifei, WANG Mengzhi

doi:10.3969/j.issn.1006-267x.2019.04.056

2019 , Vol. 31 >Issue 4: 1952 - 1960

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.056

Molecular Nutrition

Effects of Different Combinations of Silage Bacteria, Cellulolytic Enzymes and Carbon Sources on Fermentation Quality of Rice Straw Silage

Expand

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2018-09-12

Online published: 2019-04-16

Fold

Abstract

The purpose of this experiment was to study the effects of different additives on the fermentation quality of rice straw silage, and then select the most suitable additive combination for rice straw silage. This experiment is an orthogonal test included 3 factors and 3 levels, adding different silage bacteria (Bacillus licheniformis, Lactobacillus plantarum and Enterococcus faecalis), cellulolytic enzymes (Aspergillus niger, Trichoderma viride and cellulase) and carbon source (glucose, bran and starch) to freshly harvested rice straw for silage 45 days, and a blank control group was included. Group 1 was Bacillus licheniformis+Aspergillus niger+glucose, group 2 was Bacillus licheniformis+Trichoderma viride+bran, group 3 was Bacillus licheniformis+cellulase+starch, group 4 was Lactobacillus plantarum+Aspergillus niger+bran, group 5 was Lactobacillus plantarum+Trichoderma viride+starch, group 6 was Lactobacillus plantarum+cellulase+glucose, group 7 was Enterococcus faecalis+Aspergillus niger+starch, group 8 was Enterococcus faecalis+Trichoderma viride+glucose, and group 9 was Enterococcus faecalis+cellulase+bran. Samples were collected for analyzing the fermentation quality on 15, 30 and 45 days of silage. The results showed as follows:1) the mean of ammoniacal nitrogen (NH₃-N)/total nitrogen (TN) in groups 2 and 4 was significantly higher than that in blank control group (P<0.05), the mean of NH₃-N/TN in group 9 had no significant difference compared with blank control group (P>0.05), and the mean in the other groups was significantly lower than that in blank control group (P<0.05). 2) Except groups 1 and 3, the mean of lactic acid content in other groups was significantly higher than that in blank control group (P<0.05); except groups 1 and 3, the mean of acetic acid content in other groups was significantly lower than that in blank control group (P<0.05); except groups 6, 7, 8 groups, the mean of butyric acid content in blank control group was significantly higher than that in other groups (P<0.05). 3) The mean of total scores in groups 4 to 9 was significantly higher than that in blank control group and other groups (P<0.05), that in groups 1 and 3 had no significant difference compared with blank control group (P>0.05). 4) As for orthogonal analysis, the R value was silage bacteria>cellulolytic enzymes at 15, 30 and 45 days of silage. At 15 and 45 days of silage, the R value was cellulolytic enzymes>carbon sources, and Lactobacillus plantarum and Trichoderma viride were the excellent level at 15 and 30 days of silage, while bran was the excellent level at 30 and 45 days of silage. In summary, the effect on silage quality is silage bacteria > cellulolytic enzymes > carbon source, and the most suitable additives combination for rice straw silage is Lactobacillus plantarum, Trichoderma viride and bran.

Key words： silage rice straw; silage bacteria; cellulolytic enzymes; carbon source; fermentation quality; orthogonal design

Cite this article

OUNGYANG Jialiang, QI Ruxin, CHEN Yifei, WANG Mengzhi . Effects of Different Combinations of Silage Bacteria, Cellulolytic Enzymes and Carbon Sources on Fermentation Quality of Rice Straw Silage[J]. Chinese Journal of Animal Nutrition, 2019 , 31(4) : 1952 -1960 . DOI: 10.3969/j.issn.1006-267x.2019.04.056

References

[1] VAN SOEST P J.Rice straw,the role of silica and treatments to improve quality[J].Animal Feed Science and Technology,2006,130(3/4):137-171.

[2] GAO L J,YANG H Y,WANG X F,et al.Rice straw fermentation using lactic acid bacteria[J].Bioresource Technology,2008,99(8):2742-2748.

[3] 郑会超,杨金勇,应如朗,等.凋萎和添加剂对稻草裹包青贮发酵品质和营养价值的影响[J].动物营养学报,2017,29(4):1312-1318.

[4] QUEIROZ O C M,ARRIOLA K G,DANIEL J L P,et al.Effects of 8 chemical and bacterial additives on the quality of corn silage[J].Journal of Dairy Science,2013,96(9):5836-5843.

[5] BOLSEN K K,ASHBELL G,WEINBERG Z G.Silage fermentation and silage additives-review[J].Asian:Australasian Journal of Animal Sciences,1996,9(5):483-494.

[6] 宋鸽,朱小清,张诗,等.绿汁发酵液和纤维素酶对稻草青贮及稻草、甘蔗梢混合青贮品质的影响[J].中国畜牧兽医,2017,44(12):3512-3518.

[7] WANG J,CHEN L,YUAN X,et al.Effects of molasses on the fermentation characteristics of mixed silage prepared with rice straw,local vegetable by-products and alfalfa in Southeast China[J].Journal of Integrative Agriculture,2017,16(3):664-670.

[8] 卢强,于浩然,任志花,等.苜蓿青贮添加剂研究进展[J].草原与草业,2018(3):1-4.

[9] 郭海明,夏天婵,朱雯,等.青贮添加剂对稻草青贮品质和有氧稳定性的影响[J].草业学报,2017,26(2):190-196.

[10] 中国农业部.青贮饲料质量评定标准(试行)[J].饲料工业,1996(21):5-7.

[11] BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.

[12] AOAC.Official methods of analysis of AOAC international[M].18th ed.Washington,D.C.:AOAC,2005.

[13] KAWAMOTO H,OGAWA M,HAYASAKA K,et al.Effects of formic acid-propionic acid-ammonium complex on fermentation quality and palatability of wrapped round bale silage[J].Bulletin of the National Grassland Research Institute,2000(59):18-23.

[14] KHAN N A,CONE J W,HENDRIKS W H.Stability of fatty acids in grass and maize silages after exposure to air during the feed out period[J].Animal Feed Science and Technology,2009,154(3/4):183-192.

[15] 刘瑞江,张业旺,闻崇炜,等.正交试验设计和分析方法研究[J].实验技术与管理,2010,27(9):52-55.

[16] 夏友国.产纤维素酶菌株的筛选及不同添加剂在青贮饲料中的应用研究[D].硕士学位论文,福州:福建农林大学,2010.

[17] HOFVENDAHL K,HAHN-HÄGERDAL B.Factors affecting the fermentative lactic acid production from renewable resources[J].Enzyme and Microbial Technology,2000,26(2/3/4):87-107.

[18] 韩吉雨.青贮发酵体系中乳酸菌多样性的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2009.

[19] KARUNARATNE A,WEZENBERG E,BULLERMAN L B.Inhibition of mold growth and aflatoxin production by Lactobacillus spp.[J].Journal of Food Protection,1990,53(3):230-236.

[20] KUNG L,Jr,TAYLOR C C,LYNCH M P,et al.The effect of treating alfalfa with Lactobacillus buchneri 40788 on silage fermentation,aerobic stability,and nutritive value for lactating dairy cows[J].Journal of Dairy Science,2003,86(1):336-343.

[21] 葛文中,李楠.绿色木霉应用的研究进展[J].黑龙江八一农垦大学学报,2005,17(2):75-80.

[22] VISSERS M M M,DRIEHUIS F,TE GIFFEL M C,et al.Concentrations of butyric acid bacteria spores in silage and relationships with aerobic deterioration[J].Journal of Dairy Science,2007,90(2):928-936.

[23] ZHAO J,DONG Z H,LI J F,et al.Ensiling as pretreatment of rice straw:the effect of hemicellulase and Lactobacillus plantarum on hemicellulose degradation and cellulose conversion[J].Bioresource Technology,2018,266:158-165.

[24] 周健民,沈仁芳.土壤学大辞典[M].北京:科学出版社,2013.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References