Effects of Combination of Duck Oil Diglyceride and Vitamin D3 on Organ Indexes, Blood 25 Hydroxyl Vitamin D3 Content, Tibia Development Indexes and Liver Vitamin D Receptor and Peroxisome Proliferators-Activated Receptors &alpha; Gene Expressions of Obese SD Rats

LIU Yanan; WANG Baowei; GE Wenhua; ZHANG Ming; SUN Jingxin; KONG Min

doi:10.3969/j.issn.1006-267x.2020.01.055

2020 , Vol. 32 >Issue 1: 472 - 480

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.055

Effects of Combination of Duck Oil Diglyceride and Vitamin D₃ on Organ Indexes, Blood 25 Hydroxyl Vitamin D₃ Content, Tibia Development Indexes and Liver Vitamin D Receptor and Peroxisome Proliferators-Activated Receptors α Gene Expressions of Obese SD Rats

LIU Yanan ,
WANG Baowei ,
GE Wenhua ,
ZHANG Ming ,
SUN Jingxin ,
KONG Min

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1. Department of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
2. National Waterfowl Industry Technical System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2019-07-22

Online published: 2020-01-19

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Abstract

This experiment was conducted to study the effects of duck oil diglyceride and vitamin D₃ coordination on organ indexes, blood 25 hydroxyl vitamin D₃ content, tibia development indexes and liver vitamin D receptor (VDR) and peroxisome proliferators-activated receptors α (PPARα) gene expressions of obese SD rats, to explore the synergistic relationship between duck oil diglyceride and vitamin D₃. Sixty male SD rats were randomly assigned into 4 groups (control group, obese model group, vitamin D₃ group and duck oil diglyceride+vitamin D₃ group) with 3 replicates per group and 5 rats per replicate. During 1 to 3 weeks, rats in the control group were fed a basal diet, and the others in other groups were fed high-fat diets for obesity modeling. During 4 to 6 weeks, after successful modeling, rats in the control group were fed a basal diet, rats in the obese model group were fed a high-fat diet, rats in vitamin D₃ group and duck oil diglyceride+vitamin D₃ group were fed high-fat diet supplemented 12.5 μg/kg vitamin D₃, rats in duck oil diglyceride+vitamin D₃ group were given 1 mL/(kg·BW d) duck oil diglyceride by gavage, and rats in other groups were given the same amount of distilled water by gavage. The experiment lasted for 6 weeks. The results showed that compared with the obese model group:1) the combination of duck oil diacylglycerol and vitamin D₃ could significantly reduce the liver index (P<0.05), but had no significant effect on the indexes of heart, spleen and kidney (P>0.05); 2) the combination of duck oil diglyceride and vitamin D₃ could significantly increase the blood 25 hydroxy vitamin D₃ content (P<0.05); 3) the combination of duck oil diglyceride and vitamin D could significantly increase the bone density and bone strength in tibia (P<0.05), and significantly decreased the blood bone derived alkaline phosphatase activity (P<0.05); 4) the combination of duck oil diglyceride and vitamin D₃ could significantly increase the liver VDR and PPARα gene expressions (P<0.05). In conclusion, the combination of duck oil diglyceride and vitamin D₃ can decrease the liver index of obese rats, increase the blood 25 hydroxy vitamin D₃ content, increase the liver VDR and PPARα gene expressions, and promote the development of tibia.

Key words： duck oil diglyceride; vitamin D₃; tibia development; vitamin D receptor; PPARα; obese rats

Cite this article

LIU Yanan , WANG Baowei , GE Wenhua , ZHANG Ming , SUN Jingxin , KONG Min . Effects of Combination of Duck Oil Diglyceride and Vitamin D₃ on Organ Indexes, Blood 25 Hydroxyl Vitamin D₃ Content, Tibia Development Indexes and Liver Vitamin D Receptor and Peroxisome Proliferators-Activated Receptors α Gene Expressions of Obese SD Rats[J]. Chinese Journal of Animal Nutrition, 2020 , 32(1) : 472 -480 . DOI: 10.3969/j.issn.1006-267x.2020.01.055

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