Relationship between Protein Nutrition Value, Rumen Degradation Characteristics and Its Molecular Structure of Whole Silage Corn in Different Producing Areas

YAO Enyue; ZHENG Jian; FANG Xinpeng; XIN Hangshu; ZHANG Yonggen

doi:10.3969/j.issn.1006-267x.2020.02.037

2020 , Vol. 32 >Issue 2: 815 - 826

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.037

RUMINANT AND HERBIVORE NUTRITION AND FEED

Relationship between Protein Nutrition Value, Rumen Degradation Characteristics and Its Molecular Structure of Whole Silage Corn in Different Producing Areas

YAO Enyue ,
ZHENG Jian ,
FANG Xinpeng ,
XIN Hangshu ,
ZHANG Yonggen

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College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2019-08-05

Online published: 2020-02-21

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Abstract

The aim of this experiment was to explore the effects of different producing areas on the molecular structure of whole silage corn protein and its correlation with protein nutritional value and rumen degradation characteristics. This experiment used conventional chemical analysis methods combined with Cornell net carbohydrate-net protein system (CNCPS) and nylon bag technology to evaluate the nutritional value of whole silage corn in different producing areas. Fourier transform infrared spectroscopy (FTIR) technology was used to analyze the changes of molecular structure of silage corn protein, and then to explore the correlation between them. The results showed as follows:1) there were significant differences in the chemical constituents of whole silage corn in different producing areas (P<0.05); there were significant differences in non-protein nitrogen, medium-speed degradation proteins, slow-degrading proteins and unavailable proteins of the CNCPS protein fraction (P<0.05); there were also significant differences in the effective degradation rates of dry matter and crude protein (P<0.05). 2) After scanning by FTIR technology, it was found that amide band Ⅰ peak height and peak area, amide Ⅱ band peak height and peak area, total peak area, peak area ratio, peak height ratio of amide Ⅰ and Ⅱ bands, α-helix, β-fold peak height in the molecular structure of whole silage corn in different producing areas had significant effects (P<0.05). 3) Amide Ⅰ band peak height, peak area, amide Ⅱ peak height, peak area, amide Ⅰ band and Ⅱ band peak height ratio, peak area ratio and α-helix peak height could be used as predictors to predict the nutritional value and rumen degradation parameters of whole silage corn. Among them, silage corn protein molecular structure for crude protein (CP) (R²=0.81), acid detergent insoluble protein (ADICP) (R²=0.88), non-available protein (PC) (R²=0.85) and crude protein rapid degradation (CP_a) (R²=0.69) fitted the best. In summary, different producing areas have an effect on the protein components, CNCPS components, dry matter and crude protein rumen degradation parameters and protein molecular structure of whole silage corn. Moreover, there is a clear correlation between protein molecular structure and protein nutritional value, and the protein nutritional value of silage corn can be predicted by infrared spectral parameters.

Key words： different producing areas; whole silage corn; protein molecular structure; correlation

Cite this article

YAO Enyue , ZHENG Jian , FANG Xinpeng , XIN Hangshu , ZHANG Yonggen . Relationship between Protein Nutrition Value, Rumen Degradation Characteristics and Its Molecular Structure of Whole Silage Corn in Different Producing Areas[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 815 -826 . DOI: 10.3969/j.issn.1006-267x.2020.02.037

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