In order to improve the crude protein content of fermented corn straw, Trichoderma koningiopsis and Phanerochaete chrysosporium were used to ferment corn straw, and Saccharomyces cerevisiae was added when the reducing sugar content of fermented substrate was higher. The effects of inoculation time, inoculation amount and co-fermentation time with Trichoderma koningiopsis and Phanerochaete chrysosporium on crude protein content in fermented corn straw were studied. On the basis of this experiment, response surface methodology was used to further evaluate the effects of various factors and their interactions on the crude protein content of fermented corn straw. The results showed that the optimal fermentation parameters were 8.5% Saccharomyces cerevisiae inoculated on the 7th day when the corn stalk was co-fermented with Trichoderma koningii and Phanerochaete chrysosporium. After 80 h of co-fermentation, the crude protein content of corn straw could reach the maximum value of 13.23%. In conclusion, the crude protein content of corn straw fermented by Trichoderma koningiopsis, Phanerochaete chrysosporium and Saccharomyces cerevisiae is higher than that of corn straw.
WANG Zhao
,
ZHANG Zhongqing
,
JIANG Ning
,
ZHANG Aizhong
. Mixed Fermentation of Corn Straw by Trichoderma koningiopsis, Phanerochaete chrysosporium and Saccharomyces cerevisiae to Increase Crude Protein Content of Corn Straw[J]. Chinese Journal of Animal Nutrition, 2020
, 32(3)
: 1284
-1292
.
DOI: 10.3969/j.issn.1006-267x.2020.03.036
[1] 熊本海,庞之洪,罗清尧.中国饲料成分及营养价值表(2013年第24版)(续)[J].中国饲料,2013(22):38-42.
[2] STEPANOVA E V,KOROLEVA O V,VASILCHENKO L G,et al.Fungal decomposition of oat straw during liquid and solid-state fermentation[J].Applied Biochemistry and Microbiology,2003,39(1):74-84.
[3] 张阿强.混菌固态发酵玉米秸秆产单细胞蛋白饲料的研究[D].硕士学位论文.兰州:兰州交通大学,2017.
[4] 马根.黄化玉米秸秆的饲料化技术研究[D].硕士学位论文.长春:吉林农业大学,2015.
[5] 姜雪.B17菌株的鉴定及产纤维素酶的初步研究[D].硕士学位论文.哈尔滨:黑龙江八一农垦大学,2015.
[6] 陈合,余建军,舒国伟,等.多菌种分步降解玉米秸秆生产蛋白饲料的工艺[J].农业工程学报,2009,25(12):331-334.
[7] 武月雷.真菌预处理改善油茶籽渣饲用价值研究[D].硕士学位论文.杭州:浙江大学,2017.
[8] 伍文宪,刘勇,张蕾,等.拟康宁木霉的分离鉴定及其在纤维素降解中的应用研究[C]//中国植物保护学会2015年学术年会论文集.长春:中国植物保护学会,2015.
[9] CHEN Y,WANG Y Y,XU Z,et al.Enhanced humification of maize straw and canola residue during composting by inoculating Phanerochaete chrysosporium in the cooling period[J].Bioresource Technology,2019,293, 122075.DOI:10.1016/j.biortech.2019.122075.
[10] HUANG S H,HUANG D Y,WU Q T,et al.The effects of environmental C/N on the activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium[J].Pedosphere,2017,doi:10.1016/S1002-0160(17)60391-6.
[11] 卢松.微生物处理玉米秸秆的腐解特征研究[D].硕士学位论文.重庆:西南大学,2010:17-27.
[12] 张思宇.利用黑曲霉和酿酒酵母转化玉米废弃物产蛋白的研究[D].硕士学位论文.哈尔滨:哈尔滨工业大学,2017.
[13] 孙旸,地里百尔·努尔,林晓琼,等.酵母和乳酸菌混合发酵对玉米秸秆蛋白质含量的影响[J].吉林农业大学学报,2016,38(5):557-561.
[14] 张仲卿,张爱忠,姜宁.混合真菌发酵对玉米秸秆纤维素与木质素降解率的影响[J].动物营养学报,2019,31(3):1385-1395.
[15] 赵凯,许鹏举,谷广烨.3,5-二硝基水杨酸比色法测定还原糖含量的研究[J].食品科学,2008,29(8):534-536.
[16] 惠文森,王康英,申晓蓉,等.酵母菌发酵玉米秸秆试验研究[J].草业学报,2011,20(6):180-185.
[17] 马敏.添加三菌发酵秸秆饲料对生长育肥猪生产性能及肠道微生物区系的影响[D].硕士学位论文.延吉:延边大学,2016.
[18] 杨森,王石垒,张雷,等.甜高粱茎秆残渣生料多菌种固态发酵生产蛋白饲料[J].农业工程学报,2015,31(15):309-314.
[19] LU Y P,YANG B,GREGG D,et al.Cellulase adsorption and an evaluation of enzyme recycle during hydrolysis of steam-exploded softwood residues[J].Applied Biochemistry and Biotechnology,2002,98:641-654.
