Effects of Lactobacillus casei on Microbiota Composition, Lactic Acid, Short Chain Fatty Acids and Long Chain Fatty Acids Contents in Intestinal Digesta of Beijing Black Pigs during Fattening Stage

WANG Sixin; JI Haifeng; WANG Hongwei; LIU Hui; SHI Guohua; ZHANG Dongyan; WANG Jing; ZHANG Wei; WANG Yamin

doi:10.3969/j.issn.1006-267x.2020.04.017

2020 , Vol. 32 >Issue 4: 1595 - 1604

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.017

Effects of Lactobacillus casei on Microbiota Composition, Lactic Acid, Short Chain Fatty Acids and Long Chain Fatty Acids Contents in Intestinal Digesta of Beijing Black Pigs during Fattening Stage

WANG Sixin ,
JI Haifeng ,
WANG Hongwei ,
LIU Hui ,
SHI Guohua ,
ZHANG Dongyan ,
WANG Jing ,
ZHANG Wei ,
WANG Yamin

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1. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
2. Beijing Hei Liu Animal Husbandry Science and Technology Co., Ltd., Beijing 102211, China

Received date: 2019-10-10

Online published: 2020-04-16

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Abstract

This experiment was conducted to evaluate the effects of Lactobacillus casei on microbiota, lactic acid, short chain fatty acid and long chain fatty acid contents in the intestinal digesta of Beijing black pigs during fattening stage. A total of 120 healthy Beijing black fattening pigs with an average body weight of (62.77±0.59) kg were randomly allotted into 2 groups:control group and Lactobacillus casei group. Each group had 5 replicates with 12 pigs per replicate (barrow and female in half). Pigs in the control group were fed a basal diet (without antibiotics or Lactobacillus casei), and pigs in the Lactobacillus casei group were fed the basal diet supplemented with Lactobacillus casei (2.0×10⁹ CFU/kg diet). A total of 20 healthy pigs (half of the steers and sows) were selected and slaughtered at an average body weight of 92 kg, and the contents in jejunum and colon were collected and used to determine the microbiota structure and lactic acid, short chain fatty acid contents and long chain fatty acid contents. The results showed as follows:1) compared with the control group, the relative abundance of Firmicutes and Streptococcus were significantly increased (P<0.05) and the relative abundance of Proteobacteria, Terrisporobacter and Escherichia-Shigella were significantly decreased in the jejunum (P<0.05); the relative abundance of Bacteroidetes, Lactobacillus and norank_f_Bacteroidales_S24-7_group were significantly increased (P<0.05) and the relative abundance of Streptococcus was significantly decreased in the colon of Lactobacillus casei group (P<0.05). 2) Compared with the control group, the content of lactic acid was significantly decreased (P<0.05), and the content of butyric acid was significantly increased in the colon of Lactobacillus casei group (P<0.05). 3)Compared with the control group, the contents of behenic acid and Cis-15-tetracosenic acid were significantly decreased (P<0.05), and the contents of docosahexaenoic acid and n-3 polyunsaturated fatty acid were significantly increased in the jejunum (P<0.05); the contents of palmitoleic acid and arachidonic acid were significantly decreased in the colon of the Lactobacillus casei group (P<0.05). The results show that Lactobacillus casei supplementation to diets for fattening pigs can reduce the relative abundance of Escherichia-Shigella in the jejunum and increase the relative abundance of Lactobacillus in the colon; can reduce the content of lactic acid and increase the content of butyric acid in the colon; can increase the content of docosahexaenoic acid in the jejunum and decrease the content of arachidonic acid in the colon. The changes in gut are propitious to improve the health and growth performance of fattening pigs.

Key words： Beijing black pigs; Lactobacillus casei; intestinal digesta; microflora; fatty acids

Cite this article

WANG Sixin , JI Haifeng , WANG Hongwei , LIU Hui , SHI Guohua , ZHANG Dongyan , WANG Jing , ZHANG Wei , WANG Yamin . Effects of Lactobacillus casei on Microbiota Composition, Lactic Acid, Short Chain Fatty Acids and Long Chain Fatty Acids Contents in Intestinal Digesta of Beijing Black Pigs during Fattening Stage[J]. Chinese Journal of Animal Nutrition, 2020 , 32(4) : 1595 -1604 . DOI: 10.3969/j.issn.1006-267x.2020.04.017

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