Protective Effects of &beta;-Glucan from Saccharomyces cerevisiae on Lipopolysaccharide Induced Oxidative Stress Injury in Sheep Lymphocyte

YU Chunwei; LI Dongfang; LIU Shixiong; HAO Lingkui; GAO Min; HU Honglian; LIU Dacheng

doi:10.3969/j.issn.1006-267x.2020.06.051

2020 , Vol. 32 >Issue 6: 2904 - 2910

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.051

Protective Effects of β-Glucan from Saccharomyces cerevisiae on Lipopolysaccharide Induced Oxidative Stress Injury in Sheep Lymphocyte

YU Chunwei ,
LI Dongfang ,
LIU Shixiong ,
HAO Lingkui ,
GAO Min ,
HU Honglian ,
LIU Dacheng

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1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Research Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Animal Sciences, Hohhot 010031, China

Received date: 2019-12-11

Online published: 2020-06-16

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Abstract

This experiment was conducted to study the effects of β-glucan from Saccharomyces cerevisiae on the antioxidant capacity of sheep lymphocytes. Based on the establishment of lipopolysaccharide (LPS)-induced oxidative stress in sheep lymphocytes, the effects of β-glucan on cell viability were determined by MTT assay by adding different concentrations [0(control), 5, 10, 20, 50 and 100 μg/mL] of β-glucan to the culture medium. The biochemical method was used to determine the effects of β-glucan on the changes of antioxidant indexes in sheep lymphocytes. The results showed as follows: β-glucan showed no cytotoxicity after being applied to lymphocytes at a concentration of 0 to 20 μg/mL, when the concentration of β-glucan was 50 μg/mL, the cell viability decreased significantly (P<0.05); compared with the control group, the malonaldehyde (MDA) content extremely significantly increased (P<0.01), superoxide dismutase (ROS) and catalase (CAT) activities extremely significantly decreased (P<0.05), and glutathion peroxidase (GSH-Px) activity decreased extremely significantly (P<0.01) in lymphocytes treated by LPS. After adding 10 μg/mL β-glucan for 6 h, compared with the LPS group, the activity of SOD and CAT in lymphocytes extremely significantly increased (P<0.01), the activity of GSH-Px significantly increased (P<0.05), and the contents of MDA and ROS extremely significantly decreased (P<0.01). In conclusions, LPS can induce oxidative stress in sheep lymphocytes. β-glucan can inhibit LPS-induced oxidative injury of lymphocytes and has a good protective effect on LPS-induced oxidative injury of sheep lymphocytes, and there is a dose-dependent effect.

Key words： yeast β-glucan; lymphocyte; lipopolysaccharide; reactive oxygen species; antioxidant

Cite this article

YU Chunwei , LI Dongfang , LIU Shixiong , HAO Lingkui , GAO Min , HU Honglian , LIU Dacheng . Protective Effects of β-Glucan from Saccharomyces cerevisiae on Lipopolysaccharide Induced Oxidative Stress Injury in Sheep Lymphocyte[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2904 -2910 . DOI: 10.3969/j.issn.1006-267x.2020.06.051

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