Effects of Epiphytic Microorganisms on Fermentation Quality and Aerobic Stability of Silage

HUANG Feng; LI Hao; WANG Kun; ZHOU Xiaokang; GU Qichao; ZHANG Lu; ZHANG Jie; YI Siyu; ZOU Caixia

doi:10.3969/j.issn.1006-267x.2020.12.052

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 12: 5976 - 5984

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.052

Effects of Epiphytic Microorganisms on Fermentation Quality and Aerobic Stability of Silage

HUANG Feng ,
LI Hao ,
WANG Kun ,
ZHOU Xiaokang ,
GU Qichao ,
ZHANG Lu ,
ZHANG Jie ,
YI Siyu ,
ZOU Caixia

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College of Animal Science and Technology, Guangxi University, Nanning 530004, China

Received date: 2020-05-19

Online published: 2020-12-07

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Abstract

The effects of epiphytic microorganisms on silage fermentation quality and aerobic stability were studied in current experiment. Corn straw, elephant grass and sugarcane top were selected as the research objects, and obtain their epiphytic microorganisms by centrifuging the silage microbial suspension at high speed (15 000×g for 90 min). Fresh corn straw, elephant grass and sugar cane top were smashed, heated at 65 ℃ for 3 h and then heated at 105 ℃ for 15 h for sterilization. The 3 (sterilization raw materials)×3 (epiphytic microorganisms) experiment was used to design and reconstructed to a dry matter level of 40% before ensiling. The experiment was divided into 9 treatments with 3 replicates. All treatment samples were ensiled for 65 d. After silage, pH, conventional nutrient composition and volatile fatty acid content in each treatment were determined, and then subjected to an aerobic stability test for 8 days. The results showed as follows: 1) when using sterilized corn stalk as fermentation substrate, compared with the epiphytic microorganisms of corn stalk and elephant grass, the content of water-soluble carbohydrate (WSC) was significantly increased (P<0.01), increased propionic acid content (P>0.05), and the contents of lactic acid and acetic acid were significantly decreased by adding sugarcane top epiphytic microorganisms (P<0.01). 2) When using sterilized elephant grass as fermentation substrate, compared with the epiphytic microorganisms of corn stalk and elephant grass, the contents of crude protein (P<0.01) and WSC (P<0.05) were significantly increased by adding sugarcane top epiphytic microorganisms. However, the addition of corn straw and sugarcane top epiphytic microorganisms significantly increased the content of lactic acid compared with the epiphyte microorganisms of elephant grass (P<0.05). 3) When using sterilized sugarcane top as fermentation substrate, compared with elephant grass and sugarcane top epiphytic microorganisms, adding corn straw epiphytic microorganisms significantly increased lactic acid content (P<0.01). 4) The epiphytic microorganisms of corn straw could improve the aerobic stability of elephant grass silage. In conclusion, the epiphytic microorganisms of corn straw can increase the contents of lactic acid and acetic acid in silage materials, and has a positive effect on the aerobic stability of the elephant grass silage.

Key words： silage; epiphytic microorganism; fermentation quality; aerobic stability

Cite this article

HUANG Feng , LI Hao , WANG Kun , ZHOU Xiaokang , GU Qichao , ZHANG Lu , ZHANG Jie , YI Siyu , ZOU Caixia . Effects of Epiphytic Microorganisms on Fermentation Quality and Aerobic Stability of Silage[J]. Chinese Journal of Animal Nutrition, 2020 , 32(12) : 5976 -5984 . DOI: 10.3969/j.issn.1006-267x.2020.12.052

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