Effects of Clostridium butyricum and Bacillus subtilis on Growth Performance, Digestive Enzyme Activities, Antioxidant Function and Intestinal Morphology of Meat Geese

  • YU Jie ,
  • FAN Xue ,
  • ZHAO Minmeng ,
  • DONG Biao ,
  • WANG Jian ,
  • GONG Daoqing
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  • 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China

Received date: 2020-07-05

  Online published: 2021-02-04

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Abstract

This study aimed to investigate the effects of Clostridium butyricum and Bacillus subtilis on growth performance, digestive enzyme activities, antioxidant function and intestinal morphology of Yangzhou geese aged from 1 to 70 days. A total of 288 healthy one-day-old Yangzhou male geese with similar body weight were randomly divided into 4 groups with 6 replicates in each group and 12 geese in each replicate. Geese in group A (control group) were fed a basal diet, geese in group B were fed the basal diet supplemented with 250 mg/kg Clostridium butyricum (effective viable count was 3.0×106 CFU/g), geese in group C were fed the basal diet supplemented with 250 mg/kg Bacillus subtilis (effective viable count was 2.0×107 CFU/g), and geese in group D were fed the basal diet supplemented with 250 mg/kg Clostridium butyricum+250 mg/kg Bacillus subtilis. The experiment period was 10 weeks. The results showed as follows:1) the total weight gain of groups B, C and D was significantly higher than that of the control group (P<0.05), and the total feed intake of groups C and D was significantly higher than that of the control group (P<0.05). 2) The jejunal lipase activity of groups B, C and D was significantly higher than that of the control group (P<0.05). 3) The total antioxidant capacity in serum and ileal of groups C and D was significantly higher than that of the control group (P<0.05). The jejunal glutathione content of group D was significantly higher than that of the control group (P<0.05), and the jejunum total superoxide dismutase activity of groups C and D was significantly higher than that of the control group (P<0.05). The ileal glutathione peroxidase activity of group D was significantly higher than that of the control group (P<0.05). 4) The jejunal villi height/crypt depth (V/C) of group C was significantly higher than that of the control group (P<0.05). The ileal villus height of groups B, C and D was significantly higher than that of the control group (P<0.05), and the ileal V/C of groups C and D was significantly higher than that of the control group (P<0.05). It is concluded that dietary Clostridium butyricum, Bacillus subtilis and their mixture preparation can improve the total weight gain, jejunal lipase activity and antioxidant capacity of meat geese, in addition, dietary Bacillus subtilis can improve the intestinal morphology better.

Cite this article

YU Jie , FAN Xue , ZHAO Minmeng , DONG Biao , WANG Jian , GONG Daoqing . Effects of Clostridium butyricum and Bacillus subtilis on Growth Performance, Digestive Enzyme Activities, Antioxidant Function and Intestinal Morphology of Meat Geese[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 860 -868 . DOI: 10.3969/j.issn.1006-267x.2021.02.027

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