Effects of Hydrolyzable Tannins on Growth Performance, Antioxidant Capacity, Intestinal Morphology and Bacterial Diversity of Pearl Gentian Grouper (Epinephelus lanceolatus ♂×Epinephelus fuscoguttatus ♀)

  • ZHU Xufeng ,
  • HUANG Yang ,
  • HUANG Junhang ,
  • LI Guangli ,
  • ZHU Chunhua
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  • 1. Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
    2. Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Zhanjiang 524088, China;
    3. Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China;
    4. Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China

Received date: 2020-07-06

  Online published: 2021-02-04

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Abstract

The aim of this study was to investigate the effects of hydrolyzable tannins (HTs) on growth performance, antioxidant capacity, intestinal morphology and bacterial diversity of pearl gentian grouper (Epinephelus lanceolatus ♂×Epinephelus fuscoguttatus ♀). A total of 600 pearl gentian groupers with an initial body weight of (21.43±1.31) g were randomly divided into 5 groups with 3 replicates per group and 40 fish per replicate. HTs was added to a basal diet by substituting for the same level of microcrystalline cellulose at the levels of 0 (F0 group, as control group), 0.05% (F1 group), 0.10% (F2 group), 0.15% (F3 group) and 20% (F4 group), respectively. The experiment lasted for 60 days. The results showed that the weight gain rate (WGR) and specific growth rate (SGR) in F2 group were significantly higher than those in F0 group (P<0.05), and the feed conversion rate (FCR) in F2 group was significantly lower than that in F0 group (P<0.05). No significant differences were found in condition factor (CF) and survival rate (SR) among groups (P>0.05). Compared with the F0 group, the activities of alkaline phosphatase (AKP), acid phosphatase (ACP) and superoxide dismutase (SOD) and the mRNA relative expression levels of catalase (CAT), manganese superoxide dismutase (Mn-SOD) and glutathione peroxidase (GPx) in F2 and F3 groups were significantly increased (P<0.05). The intestinal villus height in F1, F2 and F3 groups and the intestinal muscle thickness in F3 group were significantly higher than those of F0 group (P<0.05). The Illumina MiSeq sequence assays showed that the Shannon index and Chao1 index of intestinal bacteria in experimental groups were higher than those in F0 group. The dominant species at the phylum level in all groups mainly included Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes. Compared with the F0 group, the proportion of Proteobacteria was decreased, whereas the proportions of Cyanobacteria, Actinobacteria and Bacteroidetes were increased in experimental groups. The principal coordinates analysis (PCoA) and UPGMA cluster analysis showed that the bacteria community in F2 and F3 groups might be quite similar, but different with F0 group. In conclusion, this present study documents that dietary supplemented with 0.10% to 0.15% HTs promotes growth performance, antioxidant capacity, improves the intestinal morphology and microbial community structure of pearl gentian grouper. The appropriate supplemental level of HTs obtained by quadratic regression equation is 0.10% based on WGR as the evaluation index.

Cite this article

ZHU Xufeng , HUANG Yang , HUANG Junhang , LI Guangli , ZHU Chunhua . Effects of Hydrolyzable Tannins on Growth Performance, Antioxidant Capacity, Intestinal Morphology and Bacterial Diversity of Pearl Gentian Grouper (Epinephelus lanceolatus ♂×Epinephelus fuscoguttatus ♀)[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 1020 -1035 . DOI: 10.3969/j.issn.1006-267x.2021.02.042

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