Effects of Adding Lactic Acid Bacteria and Molasses on Fermentation Quality and in Vitro Dry Matter Disappearance Rate of Rumex hanus by. Silage with Different Moisture Contents

  • ZHOU Xin ,
  • HUANG Qiulian ,
  • WANG Jian ,
  • ZHANG Jiabin ,
  • CAO Yang
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  • Heilongjiang Provincial Key Laboratory of Efficient Utilization of Feed Resources and Nutrition Manipulation in Cold Region, Engineering Research Center of Processing and Utilization of Grain By-Products, Ministry of Education, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2020-08-25

  Online published: 2021-03-18

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Abstract

This experiment explored the effects of adding lactic acid bacteria and molasses on the fermentation quality and the in vitro dry matter disappearance rate of Rumex hanus by. silage with different moisture contents, aiming to explore high-quality grass silage technology and efficiently use roughage resources. This experiment used Rumex hanus by. as the ensiling raw material and adopted a two-factor (moisture content×additive) completely randomized experimental design, in which the moisture content was adjusted to 65%, 60% and 55%, respectively, and treated by adding lactic acid bacteria inoculant Master-LP (LP), lactic acid bacteria inoculant Chikuso-1 (LAB-1), lactic acid bacteria inoculant Master-AC (AC), molasses (M) and no additive (C, control) during ensiling process. After 30 days of silage, the fermentation quality, nutrient contents, microbial population and in vitro dry matter disappearance rate of Rumex hanus by. silage were analyzed. The results showed as follows: 1) 60% group had the highest crude protein and crude fat contents, gas production, in vitro dry matter disappearance rate and the lowest neutral detergent fiber content, which was significantly different from the 65% and 55% groups (P<0.05). 2) Compared with the control, adding lactic acid bacteria inoculants and molasses during ensiling process significantly reduced the pH (P<0.05), and significantly increased the lactic acid content and gas production (P<0.05); the crude protein and crude fat contents of the LP group and LAB-1 group were significantly higher than those of the other groups (P<0.05); the neutral detergent fiber content of the LP group was significantly lower than that of the other groups (P<0.05); LAB-1 group had the highest gas production and in vitro dry matter disappearance rate, which were significantly higher than those of the other groups (P<0.05). Considering comprehensively, adding lactic acid bacteria inoculant Chikuso-1 or lactic acid bacteria inoculant Master-LP is beneficial to produce higher-quality Rumex hanus by. silage when the moisture content of Rumex hanus by. is adjusted to 60%.

Cite this article

ZHOU Xin , HUANG Qiulian , WANG Jian , ZHANG Jiabin , CAO Yang . Effects of Adding Lactic Acid Bacteria and Molasses on Fermentation Quality and in Vitro Dry Matter Disappearance Rate of Rumex hanus by. Silage with Different Moisture Contents[J]. Chinese Journal of Animal Nutrition, 2021 , 33(3) : 1594 -1606 . DOI: 10.3969/j.issn.1006-267x.2021.03.041

References

[1] 陈伟.食叶草的营养价值及产品开发研究进展[J].农产品加工,2018(14):63-64,67. CHEN W.Study on nutritional value and development of edible leaf weed and research progress on edible leaf weed products[J].Farm Products Processing,2018(14):63-64,67.(in Chinese)
[2] 柏绿山,杨秀丽.食叶草的研究与开发[J].中国果菜,2018,38(9):37-40. BAI L S,YANG X L.Research and development of leaf-eating grass[J].China Fruit & Vegetable.2018,38(9):37-40.(in Chinese)
[3] 杨秀丽,柏绿山.食叶草植物蛋白原料及其在化妆品、洗护用品中的用途:中国,201810455910.7[P].2018-05-14. YANG X L,BAI L S.Leaf-eating grass protein raw material and its use in cosmetics and toiletries:China,201810455910.7[P].2018-05-14.(in Chinese)
[4] 柏绿山,杨秀丽.食叶草功能性茶叶及其制备方法:中国,201710075060.3[P].2017-02-13. BAI L S,YANG X L,Leaf-eating grass functional tea and its preparation method:China,201710075060.3[P].2017-02-13.(in Chinese)
[5] 许庆方.影响苜蓿青贮品质的主要因素及苜蓿青贮在奶牛日粮中应用效果的研究[D].博士学位论文.北京:中国农业大学,2005. XU Q F.Studies of the factors of affecting alfalfa silage quality and the utilization of alfalfa silage in dairy cows diet[D].Ph.D.Thesis.Beijing:China Agricultural University,2005.(in Chinese)
[6] 董志浩,原现军,闻爱友,等.添加乳酸菌和发酵底物对桑叶青贮发酵品质的影响[J].草业学报,2016,25(6):167-174. DONG Z H,YUAN X J,WEN A Y,et al.Effect of lactic acid bacteria and fermentation substrates on the quality of mulberry (Morus alba) leaf silage[J].Acta Prataculturae Sinica,2016,25(6):167-174.(in Chinese)
[7] 吴金彩,刘婷婷,岳春旺,等.添加不同水平糖蜜对全株"张杂谷"青贮体外瘤胃发酵特性和营养物质降解率的影响[J].中国畜牧兽医,2018,45(10):2752-2760. WU J C,LIU T T,YUE C W,et al.Effects of different molasses levels on in vitro rumianl fermentation characteristics and nutrient degradation of whole "Zhang Hybrid Millet" silage[J].China Animal Husbandry & Veterinary Medicine,2018,45(10):2752-2760.(in Chinese)
[8] CAO Y,TAKAHASHI T,HORIGUCHI K I,et al.Effect of adding lactic acid bacteria and molasses on fermentation quality and in vitro ruminal digestion of total mixed ration silage prepared with whole crop rice[J].Grassland Science,2010,56(1):19-25.  
[9] 刘建新,杨振海,叶均安,等.青贮饲料的合理调制与质量评定标准[J].饲料工业,1999(3):3-5. LIU J X,YANG Z H,YE J A,et al.Reasonable preparation and quality evaluation standard of silage[J].Feed Industry,1999(3):3-5.(in Chinese)
[10] AOAC.Official methods of analysis of AOAC International[S].15th ed.Artinqton:Association of Official Analytical Chemists,1990.
[11] MCDONALD P,HENDERSON A R.Determination of water-soluble carbohydrates in grass[J].Journal of the Science of Food and Agriculture,1964,15(6):395-398.  
[12] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.  
[13] CAI Y,BENNO Y,OGAWA M,et al.Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage[J].Journal of Dairy Science,1999,82(3):520-526.  
[14] CAO Y,ZANG Y Q,JIANG Z H,et al.Fermentation quality and nutritive value of fresh and fermented total mixed rations containing Chinese wildrye or corn stover[J].Grassland Science,2016,62(4):213-223.  
[15] CAO Y,CAI Y M,HIRAKUBO T,et al.Fermentation characteristics and microorganism composition of total mixed ration silage with local food by-products in different seasons[J].Animal Science Journal,2011,82(2):259-266.  
[16] MCDOUGALL E I.Studies on ruminant saliva.1.The composition and output of sheep's saliva[J].Biochemical Journal,1948,43(1):99-109.  
[17] 汤少勋,姜海林,周传社,等.不同牧草品种对体外发酵产气特性的影响[J].草业学报,2005,14(3):72-77. TANG S X,JIANG H L,ZHOU C S,et al.Effects of different forage species on in vitro gas production characteristics[J].Acta Prataculturae Sinica,2005,14(3):72-77.(in Chinese)
[18] 贾鹏禹,孙蕊,李井春,等.离子排斥色谱法测定瘤胃液中乳酸和挥发性脂肪酸的含量[J].黑龙江畜牧兽医,2017(3):43-46. JIA P Y,SUN R,LI J C,et al.Determination of the contents of lactic acid and volatile fatty acids in rumen liquid using ion exclusion chromatography[J].Heilongjiang Animal Science and Veterinary Medicine,2017(3):43-46.(in Chinese)
[19] 余伯良.发酵饲料生产与应用新技术[M].北京:中国农业出版社,1999:32. YU B L.Fermented feed production and application of new technologies[M].China Agriculture Press,1999:32.(in Chinese)
[20] MUCK R.Recent advances in silage microbiology[J].Agricultural and Food Science,2013,22(1):3-15.  
[21] 周莉.苜蓿发酵品质的影响因素研究[J].饲料研究,2019,42(9):118-121. ZHOU L.Research on influencing factors of alfalfa fermentation quality[J].Feed Research,2019,42(9):118-121.(in Chinese)
[22] SHAO T,ZHANG Z X,SHIMOJO M,et al.Comparison of fermentation characteristics of Italian ryegrass (Lolium multiflorum Lam.) and guineagrass (Panicum maximum Jacq.) during the early stage of ensiling[J].Asian-Australasian Journal of Animal Sciences,2005,18(12):1727-1734.  
[23] 刘玲,陈新,李振,等.含水量及添加剂对高冰草青贮饲料品质的影响[J].草业学报,2011,20(6):203-207. LIU L,CHEN X,LI Z,et al.Effects of moisture content and additives on the quality of Agropyron elongatum silage[J].Acta Prataculturae Sinica,2011,20(6):203-207.(in Chinese)
[24] 高海娟,刘泽东,孙蕊.添加剂、含水量及贮藏时间对苜蓿青贮的影响[J].中国饲料,2020(13):35-39. GAO H J,LIU Z D,SUN R.Effects of additives,water content and storage time on alfalfa silage[J].China Feed,2020(13):35-39.(in Chinese)
[25] CAO Y,CAI Y,TAKAHASHI T,et al.Effect of lactic acid bacteria inoculant and beet pulp addition on fermentation characteristics and in vitro ruminal digestion of vegetable residue silage[J].Journal of Dairy Science,2011,94(8):3902-3912.  
[26] 王亚芳,姜富贵,成海建,等.不同青贮添加剂对全株玉米青贮营养价值、发酵品质和瘤胃降解率的影响[J].动物营养学报,2020,32(6):2765-2774. WANG Y F,JIANG F G,CHENG H J,et al.Effects of different silage additives on nutritional value,fermentation quality and rumen degradability of whole corn silage[J].Chinese Journal of Animal Nutrition,2020,32(6):2765-2774.(in Chinese)
[27] 李苗苗,靳思玉,王立超,等.不同温度下添加乳酸菌对油莎草青贮品质及体外干物质消失率的影响[J].动物营养学报,2020,32(2):827-835. LI M M,JIN S Y,WANG L C,et al.Effects of lactic acid bacteria addition on fermentation quality and dry matter digestibility of Cyperus esculentus silage under different temperatures[J].Chinese Journal of Animal Nutrition,2020,32(2):827-835.(in Chinese)
[28] SHEPERD A C,KUNG L,Jr.Effects of an enzyme additive on composition of corn silage ensiled at various stages of maturity[J].Journal of Dairy Science,1996,79(10):1767-1773.  
[29] 魏化敬.提高紫花苜蓿与多年生黑麦草、苇状羊茅混合青贮发酵品质的研究[D].博士学位论文.南京:南京农业大学,2011. WEI H J.Studies on improving the fermentation quality of mixed silages of alfalfa with perennial ryegrass or tall fescue[D].Ph.D.Thesis.Nanjing:Nanjing Agricultural University,2011.(in Chinese)
[30] 刘婷婷,张振威,岳春旺,等.添加糖蜜和青贮添加剂对"张杂谷"全株青贮发酵品质的影响[J].中国畜牧兽医,2017,44(5):1382-1387. LIU T T,ZHANG Z W,YUE C W,et al.The Effects of added molasses and additives on fermentation quality of "Zhang Hybrid Millet" whole plant silage[J].China Animal Husbandry & Veterinary Medicine,2017,44(5):1382-1387.(in Chinese)
[31] YUAN X J,YU C Q,SHIMOJO M,et al.Improvement of fermentation and nutritive quality of straw-grass silage by inclusion of wet hulless-barley distillers' grains in Tibet[J].Asian-Australasian Journal of Animal Sciences,2012,25(4):479-85.  
[32] 蔡义民,熊井清雄,廖芷,等.乳酸菌剂对青贮饲料发酵品质的改善效果[J].中国农业科学,1995,28(2):73-82. CAI Y M,KUMAI S,LIAO Z,et al.Effect of lactic acid bacteria inoculants on fermentative quality of silage[J].Scientia Agricultura Sinica,1995,28(2):73-82.(in Chinese)
[33] 张增欣,邵涛.丙酸对多花黑麦草青贮发酵动态变化的影响[J].草业学报,2009,18(2):102-107. ZHANG Z X,SHAO T.The effect of propionic acid addition on the dynamic fermentation changes of Italian[J].Acta Prataculturae Sinica,2009,18(2):102-107.(in Chinese)
[34] 郭玉琴,何欣,孙晓利.不同含水率对苜蓿青贮营养成分的影响[J].当代畜牧,2005(12):38-39. GUO Y Q,HE X,SUN X L.The effect of different moisture content on the nutritional components of alfalfa silage[J].Contemporary Animal Husbandry,2005(12):38-39.(in Chinese)
[35] 陶莲,孙启忠,玉柱,等.乳酸菌添加剂对全株玉米和苜蓿青贮品质的影响[J].中国奶牛,2009(2):13-16. TAO L,SUN Q Z,YU Z,et al.Effect of LAB additives on the quality of whole crop corn and alfalfa silages[J].China Dairy Cattle,2009(2):13-16.(in Chinese)
[36] SILVA V P,PEREIRA O G,LEANDRO E S,et al.Effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical conditions[J].Journal of Dairy Science,2016,99(3):1895-1902.  
[37] 靳思玉,王立超,李苗苗,等.添加糖蜜对油莎草青贮发酵品质及黄酮的影响[J].中国乳品工业,2020,48(3):31-37. JIN S Y,WANG L C,LI M M,et al.Effect of adding molasses on fermentation quality and flavone of oil sedge silage[J].China Dairy Industry,2020,48(3):31-37.(in Chinese)
[38] YAHAYA M S,KIMURA A,HARAI J,et al.Evaluation of structural carbohydrates losses and digestibility in alfalfa and orchardgrass during ensiling[J].Asian-Australasian Journal of Animal Sciences,2001,14(12):1701-1704.  
[39] 刘凯丽.不同含水量及添加剂对杂交构树青贮饲料品质的影响[D].硕士学位论文.晋中:山西农业大学,2019. LIU K L.Effects of different water content and additives on quality of hybrid paper mulberry silage[D].Master's Thesis.Jinzhong:Shanxi Agricultural University,2019.(in Chinese)
[40] SRINIVAS B,GUPTA B N.Rumen fermentation,bacterial and total volatile fatty acid (TVFA) production rates in cattle fed on urea-molasses-mineral block licks supplement[J].Animal Feed Science and Technology,1997,65(1/2/3/4):275-286.(in Chinese)
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