Effects of Different Vitamin C Sources on Growth Performance, Antioxidant Capacity and Immunity of Genetically Improved Farmed Tilapia

  • GONG Fulai ,
  • LIN Xue ,
  • WANG Hongquan
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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Guangzhou Tanke Bio-Tech Co., Ltd., Guangzhou 510896, China

Received date: 2020-09-06

  Online published: 2021-04-15

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Abstract

The purpose of this experiment was to investigate the effects of different vitamin c sources on growth performance, antioxidant capacity and immunity of genetically improved farmed tilapia (GIFT). A total of 500 GIFT with the initial body weight of (21.18±0.24) g was randomly divided into 4 groups with 5 replicates and replicate had 25 fish. A basal diet (non-supplemented with vitamin C) was formulated as the diet of control group. In 3 vitamin C addition groups, common vitamin C, vitamin C phosphate and zinc chelated vitamin C with the addition level of 100 mg/kg (calculated by vitamin C) were added in the basal diet, respectively. The experiment lasted for 63 days. The results showed as follows:1) the zinc chelated vitamin C group had the highest final average body weight and weight gain rate, which were significantly higher than those of the control group (P<0.05), as well as the final average body weight of the vitamin C phosphate group. The specific growth rate of the zinc chelated vitamin C group was significantly increased (P<0.05), while the feed conversion rate was significantly reduced compared with the control group and common vitamin C group (P<0.05). 2) The serum total glutathione (T-GSH) content of the vitamin C phosphate group and zinc chelated vitamin C group was significantly higher than that of the control group and common vitamin C group (P<0.05). Compared with the control group, the serum total antioxidant capacity (T-AOC) of the zinc chelated vitamin C group was significantly increased (P<0.05), and the serum malondialdehyde (MDA) content of the vitamin C phosphate group was significantly reduced (P<0.05). The serum complement 3 (C3) and complement 4 (C4) contents of the zinc chelated vitamin C group were significantly lower than those of the vitamin C phosphate group (P<0.05). The serum lysozyme (LSZ) activity of the control group was significantly lower than that of the vitamin C addition groups (P<0.05). And the serum alkaline phosphatase (AKP) activity of the vitamin C phosphate group was significantly higher than that of the control group, common vitamin C group and zinc chelated vitamin C group (P<0.05). 3) The liver MDA content of the vitamin C phosphate group was significantly lower than that of the control group, common vitamin C group and zinc chelated vitamin C group (P<0.05). And the liver isocitrate dehydrogenase (ICDH) activity of the zinc chelated vitamin C group was significantly higher than that of the control group, common vitamin C group and vitamin C phosphate group (P<0.05). In conclusion, dietary added with zinc chelated vitamin C can improve the antioxidant capacity and growth performance of GIFT, and added with vitamin C phosphate can improve the antioxidant capacity and immunity of GIFT.

Cite this article

GONG Fulai , LIN Xue , WANG Hongquan . Effects of Different Vitamin C Sources on Growth Performance, Antioxidant Capacity and Immunity of Genetically Improved Farmed Tilapia[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2378 -2389 . DOI: 10.3969/j.issn.1006-267x.2021.04.055

References

[1] 秦启伟,吴灶和,周永灿,等.饵料维生素C对青石斑鱼的非特异性免疫调节作用[J].热带海洋学报,2000,19(1):58-63. QIN Q W,WU Z H,ZHOU Y C,et al.Non-specific immunomodulatory effects of dietary vitamin C on grouper Epinephelus awoara[J].Journal of Tropical Oceanography,2000,19(1):58-63.(in Chinese)
[2] ZHAO Y,ZHAO J X,ZHANG Y,et al.Effects of different dietary vitamin C supplementations on growth performance,mucus immune responses and antioxidant status of loach (Misgurnus anguillicaudatus Cantor) juveniles[J].Aquaculture Research,2017,48(8):4112-4123.  
[3] 明建华,谢骏,徐跑,等.大黄素、维生素C及其配伍对团头鲂生长、生理生化指标、抗病原感染以及两种HSP70s mRNA表达的影响[J].水产学报,2010,34(9):1447-1459. MING J H,XIE J,XU P,et al.Effects of emodin,vitamin C and their combination on growth,physiological and biochemical parameters,disease resistance and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala)[J].Journal of Fisheries of China,2010,34(9):1447-1459.(in Chinese)
[4] 王文辉,王吉桥,程鑫,等.不同剂型维生素C对黄颡鱼生长和几种免疫指标的影响[J].中国水产科学,2006,13(6):951-958. WANG W H,WANG J Q,CHENG X,et al.Effects of dietary vitamin C levels and sources on growth and immunity of juvenile yellow catfish Pelteobagrus fulvidraco (Richardson)[J].Journal of Fishery Sciences of China,2006,13(6):951-958.(in Chinese)
[5] 金国雄,姚春凤.罗非鱼营养需求研究进展[J].广东饲料,2014,23(1):40-44. JIN G X,YAO C F.Research progress on nutritional requirements of tilapia[J].Guangdong Feed,2014,23(1):40-44.(in Chinese)
[6] 郝淑贤,李来好,杨贤庆,等.5种罗非鱼营养成分分析及评价[J].营养学报,2007,29(6):614-615,618. HAO S X,LI L H,YANG X Q,et al.Analysis and evaluation of nutrient composition of Tilapias[J].Acta Nutrimenta Sinica,2007,29(6):614-615.(in Chinese)
[7] 王万东.吉富罗非鱼养殖技术[J].养殖与饲料,2007,20(11):13-16. WANG W D.GIFT tilapia farming techniques[J].Animals Breeding and Feed,2007,20(11):13-16.(in Chinese)
[8] 张馨馨.罗非鱼产业升级路在何方?[J].中国水产,2019(7):10-11. ZHANG X X.Where is the tilapia industry upgrading?[J].China Fisheries,2019(7):10-11.(in Chinese)
[9] 于秀娟,徐乐俊,吴反修.2020中国渔业统计年鉴[M].北京:中国农业出版社,2020. YU X J,XU L J,WU F X.China fishery statistical yearbook[M].Beijing:China Agricultural Press,2020.(in Chinese)
[10] FALCON D R,BARROS M M,PEZZATO L E,et al.Physiological responses of Nile tilapia,Oreochromis niloticus,fed vitamin C-and lipid-supplemented diets and submitted to low-temperature stress[J].Journal of the World Aquaculture Society,2007,38(2):287-295.  
[11] EL-SAYED Y S,EL-GAZZAR A M,EL-NAHAS A F,et al.Vitamin C modulates cadmium-induced hepatic antioxidants' gene transcripts and toxicopathic changes in Nile tilapia,Oreochromis niloticus[J].Environmental Science and Pollution Research,2016,23(2):1664-1670.  
[12] 宋学宏,蔡春芳,潘新法,等.用生长和非特异性免疫力评定异育银鲫维生素C需要量[J].水产学报,2002,26(4):351-356. SONG X H,CAI C F,PAN X F,et al.Determining the vitamin C requirement of allogynogenetic silver crucian carp with growth and non-specific immunity[J].Journal of Fisheries of China,2002,26(4):351-356.(in Chinese)
[13] 冷向军,王道尊,李小勤.青鱼鱼种饲料中不同剂型维生素C适宜添加量的研究[J].四川农业大学学报,2002,20(2):141-143. LENG X J,WANG D Z,LI X Q.A study of proper addition of different style Vc in fingerling black carp diet[J].Journal of Sichuan Agricultural University,2002,20(2):141-143.(in Chinese)
[14] 王吉桥,王文辉,李文宽,等.饲料蛋白质和维生素C含量对黄颡鱼生长和免疫力的影响[J].水产学报,2005,29(4):512-518. WANG J Q,WANG W H,LI W L,et al.Effects of dietary protein and vitamin C levels on growth and immunity of juvenile Pelteobagrus fulvidraco[J].Journal of Fisheries of China,2005,29(4):512-518.(in Chinese)
[15] 王红权,李瑞,金柏涛,等.饲料中添加核苷酸对草鱼生长性能、体成分及血清生化指标的影响[J].动物营养学报,2019,31(6):2745-2751. WANG H Q,LI R,JIN B T,et al.Effects of dietary nucleotides on growth performance,body composition and serum biochemical parameters of grass carp[J].Chinese Journal of Animal Nutrition,2019,31(6):2745-2751.(in Chinese)
[16] 胡志洲,黄忠志,廖朝兴.草鱼早期生长阶段对维生素C的需要[J].淡水渔业,1988(2):12-14. HU Z Z,HUANG Z Z,LIAO C X.The need for vitamin C in the early growth stage of grass carp[J].Freshwater Fisheries,1988(2):12-14.(in Chinese)
[17] 吴凡,文华,蒋明,等.饲料维生素C水平对吉富罗非鱼生长性能、肌肉品质和抗氧化功能的影响[J].中国水产科学,2015,22(1):79-87. WU F,WEN H,JIANG M,et al.Effects of dietary vitamin C on growth performance,flesh quality and antioxidant function in genetically improved farmed tilapia[J].Journal of Fishery Sciences of China,2015,22(1):79-87.(in Chinese)
[18] 陈建明,叶金云,潘茜,等.饲料中添加维生素C对翘嘴鲌鱼种生长及组织中抗坏血酸含量的影响[J].中国水产科学,2007,14(1):106-112. CHEN J M,YE J Y,PAN Q,et al.Effects of vitamin C supplement in diet on growth performance and tissue ascorbic acid levels of Culter alburnus fingerlings[J].Journal of Fishery Sciences of China,2007,14(1):106-112.(in Chinese)
[19] WILSON R P,POE W E,ROBINSON E H.Evaluation of L-ascorbyl-2-polyphosphate (AsPP) as a dietary ascorbic acid source for channel catfish[J].Aquaculture,1989,81(2):129-136.  
[20] 石文雷,陆茂英,徐增洪,等.三种VC产品对草鱼鱼种生长影响的研究[J].浙江海洋学院学报(自然科学版),2001(增刊1):127-129. SHI W L,LU M Y,XU Z H,et al.Studies on the effect of three products of vitamin C on the growth performance of grass carp fingerling[J].Journal of Zhejiang Ocean University (Natural Science Edition),2001(Suppl.1):127-129.(in Chinese)
[21] 宋进美,龙广怀,张玉荣,等.不同剂型维生素C对罗非鱼生长的影响[J].水生态学杂志,1996(5):36-38. SONG J M,LONG G H,ZHANG Y R,et al.Effects of different dosage forms of vitamin C on the growth of tilapia[J].Journal of Hydroecology,1996(5):36-38.(in Chinese)
[22] 胡斌,李小勤,冷向军,等.饲料VC对草鱼生长、肌肉品质及非特异性免疫的影响[J].中国水产科学,2008,15(5):794-800. HU B,LI X Q,LENG X J,et al.Effects of dietary vitamin C on growth,meat quality and non-specific immunity indices of grass carp,Ctenopharyngodon idellus[J].Journal of Fishery Sciences of China,2008,15(5):794-800.(in Chinese)
[23] IBIYO L M O,ATTEH J O,OMOTOSHO J S,et al.Vitamin C (ascorbic acid) requirements of Heterobranchus longifilis fingerlings[J].African Journal of Biotechnology,2010,6(13):1559-1567.
[24] 万金娟.维生素C对团头鲂幼鱼生长及免疫的影响[D].硕士学位论文.南京:南京农业大学,2012. WAN J J.Effects of dietary vitamin C levels on growth and immunity of juvenile Wuchang bream[D].Master's Thesis. Nanjing:Nanjing Agricultural University,2012.(in Chinese)
[25] 翟晓虎,杨海锋,陈慧英,等.丙二醛的毒性作用及检测技术研究进展[J].上海农业学报,2018,34(1):144-148. ZHAI X H,YANG H F,CHEN H Y,et al.Research progress in the toxicity and determination technology of malondialdehyde[J].Acta Agriculturae Shanghai,2018,34(1):144-148.(in Chinese)
[26] 姚仕彬,叶元土,蔡春芳,等.丙二醛对离体草鱼肠道黏膜细胞的损伤作用[J].水生生物学报,2015,39(1):133-141. YAO S B,YE Y T,CAI C F,et al.Damage of MDA on intestinal epithelial cells in vitro of grass carp[J].Acta Hydrobiologica Sinica,2015,39(1):133-141.(in Chinese)
[27] 池磊.不同来源的维生素C对幼建鲤消化、免疫和抗氧化功能影响的比较研究[D].硕士学位论文.成都:四川农业大学,2009. CHI L.Effects of vitamin C (ethylcellulose-coated ascorbic acid and ascorbyl polyphosphate) on growth performance,functions of digestion,absorption,immune and antioxidative of juvenile Jian carp[D].Master's Thesis.Chengdu:Sichuan Agricultural University,2009. (in Chinese)
[28] 周艳玲,孙育平,黄燕华,等.饲料中添加谷胱甘肽对黄颡鱼幼鱼组织谷胱甘肽含量、免疫及抗氧化性能的影响[J].水生生物学报,2018,42(4):728-735. ZHOU Y L,SUN Y P,HUANG Y H,et al.Effects of dietary supplementation with glutathione on tissue GSH level,immunity,and antioxidant capacity of juvenile yellow catfish[J].Acta Hydrobiologica Sinica,2018,42(4):728-735.(in Chinese)
[29] LORO V L,JORGE M B,DA SILVA K R,et al.Oxidative stress parameters and antioxidant response to sublethal waterborne zinc in a euryhaline teleost Fundulus heteroclitus:protective effects of salinity[J].Aquatic Toxicology,2012,110/111:187-193.
[30] 王文博,汪建国,李爱华,等.拥挤胁迫后鲫鱼血液皮质醇和溶菌酶水平的变化及对病原的敏感性[J].中国水产科学,2004,11(5):408-412. WANG W B,WANG J G,LI A H,et al.Changges of cortisol and lysozyme levels in Carassius auratus blood after crowding stress and the fish sensitivity to pathogen[J].Journal of fishery Sciences of China,2004,11(5):408-412.(in Chinese)
[31] 卢彤岩,郭德文,赵吉伟,等.哲罗鱼不同组织SOD、CAT、ACP和AKP活力的比较研究[J].水产学杂志,2010,23(4):10-13. LU T Y,GUO D W,ZHAO J W,et al.Activity comparison of SOD,CAT,ACP and AKP in the tissues of Hucho taimen[J].Chinese Journal Fisheries,2010,23(4):10-13.(in Chinese)
[32] 付志欢,林雪,舒绪刚,等.不同锌源对吉富罗非鱼生长性能、血清生化指标、血清和肝胰脏中微量元素含量的影响[J].动物营养学报,2019,31(8):3690-3698. FU Z H,LIN X,SHU X G,et al.Effects of different zinc sources on growth performance,serumbiochemical indices and trace element contents in serum and hepatopancreas of genetic improvement of farmed tilapia[J].Chinese Journal of Animal Nutrition,2019,31(8):3690-3698.(in Chinese)
[33] 邵彦翔,陈超,张廷廷,等.高温胁迫对2种杂交石斑鱼存活率及血清生化指标的影响[J].广东海洋大学学报,2017,37(6):89-95. SHAO Y X,CHEN C,ZHANG T T,et al.Influence of high temperature stress on survival rate and serum biochemical indexes of 2 epinephelus hybrids[J].Journal of Guangdong Ocean University,2017,37(6):89-95.(in Chinese)
[34] 李芹,唐洪玉.中华倒刺鲃仔稚鱼期消化酶及碱性磷酸酶活性变化的研究[J].水生态学杂志,2010,3(5):82-85. LI Q,TANG H Y.Development of some digestive enzymes and alkaline phosphatase activities in Spinibarbus snensis larvae and juveniles[J].Journal of Hydroecology,2010,3(5):82-85.(in Chinese)
[35] 张道波,李爱杰,王如才.牙鲆对维生素C需求量的研究[J].饲料工业,2002,23(5):37-38. ZHANG D B,LI A J,WANG R C.Study on vitamin C requirement of flounder[J].Feed Industry,2002,23(5):37-38.(in Chinese)
[36] 梁俊平,张静,覃宝利,等.维生素C对氨氮胁迫下大鳞鲤幼鱼存活及鳃抗氧化酶活性的影响[J].河南农业科学,2020,49(1):157-164. LIANG J P,ZHANG J,QIN B L,et al.Effects of vitamin C on the survival and antioxidant enzyme activities of juvenile Barbus capito under ammonia-N stress[J].Journal of Henan Agricultural Sciences,2020,49(1):157-164.(in Chinese)
[37] 王红权.基于GCRV抗性提高的草鱼免疫特性分析[D].博士学位论文.长沙:湖南农业大学,2014. WANG H Q.Immuune characteristics of grass carp to improve the resistance of GCRV[D].Ph.D.Thesis.Changsha:Hunan Agricultural University,2014.(in Chinese)
[38] 钱沛锋.维生素C对胡子鲶免疫机能、生长性能和抗病力影响的研究[D].硕士学位论文.广州:华南农业大学,2004. QIAN P F.Effects of vitamin C on immune function,growth performance and disease resistance of bearded catfish[D].Master's Thesis.Guangzhou:South China Agricultural University,2004.(in Chinese)
[39] 李桂峰,钱沛锋,孙际佳,等.维生素C对胡子鲶血清免疫相关酶活性的影响[J].大连海洋大学学报,2004,19(4):301-305. LI G F,QIAN P F,SUN J J,et al.Effects of vitamin C on enzymes activities related to immunity in Claris batrachus serum[J].Journal of Dalian Ocean University,2004,19(4):301-305.(in Chinese)
[40] 王秀英.饵料维生素C对黑鲷仔鱼生长和体组织生化指标的影响[D].硕士学位论文.杭州:浙江大学,2004. WANG X Y.Effects of dietary vitamin C on growth performance and tissue biochemical index of juvenile black seabream (Sparus macrocephalus)[D].Master's Thesis.Hangzhou:Zhejiang University,2004.(in Chinese)
[41] 申树芳,张英锋.异柠檬酸脱氢酶[J].化学教育,2016,37(2):2-5. SHEN S F,ZHANG Y F.Isocitrate dehydrogenase[J].Chinese Journal of Chemical Education,2016,37(2):2-5.(in Chinese)
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