POULTRY NUTRITION AND FEED

Effects of Vitamin A and Vitamin K3 on Performance, Egg Quality, Liver Index and Lipid Metabolism of Laying Hens during Later Laying Period

  • XI Yu ,
  • FANG Bijun ,
  • XU Jingyun ,
  • ZHAO Di ,
  • GUO Shuangshuang ,
  • ZHANG Zhengfan ,
  • DING Binying
Expand
  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2020-12-13

  Online published: 2021-07-06

Supported by

 

Abstract

This experiment was conducted to study the effects of vitamin A and vitamin K3 on performance, egg quality, liver index and lipid metabolism of laying hens during later laying period. In a 3×3 factorial arrangement, a total of 1 080 Roman laying hens (88-week-old) were allotted into 9 treatments with 3 replicates per treatment and 15 hens per replicate. Dietary vitamin A supplemental levels were 0, 7 000 and 14 000 IU/kg, respectively, and vitamin K3 supplemental levels were 0, 2.0 and 4.0 mg/kg, respectively. The experiment lasted for 8 weeks. The results showed as follows:1) Vitamin A and vitamin K3 had no significant effects on performance and egg quality of laying hens during later laying period (P>0.05). 2) Compared with 0 IU/kg vitamin A supplementation, the 7 000 and 14 000 IU/kg vitamin A supplementation significantly increased the contents of high-density lipoprotein and very low-density lipoprotein in serum at week 4 (P<0.05), and significantly decreased the serum triglyceride (TG) content and liver index at week 8 (P<0.05).; compared with 0 and 2.0 mg/kg vitamin K3 supplementation, the 4.0 mg/kg vitamin K3 supplementation significantly decreased the serum TG content at week 8 (P<0.05). 3) Compared with 7 000 IU/kg vitamin A supplementation, the 14 000 IU/kg vitamin A supplementation significantly decreased the contents of total cholesterol and TG in liver (P<0.05). 4) Compared with 0 IU/kg vitamin A supplementation, the 14 000 IU/kg vitamin A supplementation significantly down-regulated the mRNA relative expression levels of peroxisome proliferator activated receptor γ and fatty acid synthetase in liver (P<0.05), and significantly up-regulated the liver sodium glucose cotransporter 1 mRNA relative expression level (P<0.05). 5) Vitamin A and vitamin K3 had significant interaction on serum TG content and liver index (P<0.05). In conclusion, dietary supplementation of 14 000 IU/kg vitamin A and 4.0 mg/kg vitamin K3 can improve the function of lipid metabolism of laying hens during later laying period, decrease the liver index, but it does not affect the performance and egg quality.

Cite this article

XI Yu , FANG Bijun , XU Jingyun , ZHAO Di , GUO Shuangshuang , ZHANG Zhengfan , DING Binying . Effects of Vitamin A and Vitamin K3 on Performance, Egg Quality, Liver Index and Lipid Metabolism of Laying Hens during Later Laying Period[J]. Chinese Journal of Animal Nutrition, 2021 , 33(7) : 3863 -3872 . DOI: 10.3969/j.issn.1006-267x.2021.07.028

References

[1] GAN L P,ZHAO Y Z,MAHMOOD T,et al.Effects of dietary vitamins supplementation level on the production performance and intestinal microbiota of aged laying hens[J].Poultry Science,2020,99(7):3594-3605.  
[2] LITWAC K G.Vitamins and the immune system[J].Elsevier Ltd Oxford,2011,17(1):455-456.
[3] CHEN F,JIANG Z Y,JIANG S Q,et al.Dietary vitamin A supplementation improved reproductive performance by regulating ovarian expression of hormone receptors,caspase-3 and Fas in broiler breeders[J].Poultry Science,2016,95(1):30-40.  
[4] TOKITA H,TSUCHIDA A,MIYAZAWA K,et al.Vitamin K2-induced antitumor effects via cell-cycle arrest and apoptosis in gastric cancer cell lines[J].International Journal of Molecular Medicine,2006,17(2):235-243.
[5] 夏兆飞,蒋国文,潘庆山,等.大剂量维生素A、K及其互作对AA肉鸡骨骼的影响[J].中国兽医杂志,1997,23(2):13-15. XIA Z F,JIANG G W.Effects of high dose vitamin A,K and their interaction on bone of AA broilers[J].Chinese Journal of Veterinary Medicine,1997,23(2):13-15.(in Chinese)
[6] 李慧莲,王晓洁,张养东,等.维生素A在脂质过氧化和抗氧化功能上的研究进展[J].饲料博览,2008(9):24-26. LI H L,WANG X J,ZHANG Y D.Research progress of vitamin A on lipid peroxidation and antioxidant function[J].Feed Review,2008(9):24-26.(in Chinese)
[7] KNAPEN M H J,JARDON K M,VERMEER C.Vitamin K-induced effects on body fat and weight:results from a 3-year vitamin K2 intervention study[J].European Journal of Clinical Nutrition,2018,72(1):136-141.  
[8] 夏兆飞,金久善.大剂量维生素A、K及其互作对AA肉鸡生产性能和凝血机能的影响[J].中国兽医学报,2005,25(1):104-107. XIA Z F,JIN J S.Effects of high dose vitamin A,K and their interaction on production performance and coagulation function of AA broilers[J].Chinese Journal of Veterinary Medicine,2005,25(1):104-107.(in Chinese)
[9] 郝二英,黄晨轩,孙浩政,等.外源褪黑素对产蛋后期蛋鸡生产性能、血清指标及体内褪黑素含量的影响[J].动物营养学报,2020,32(5):177-188. HAO E Y,HUANG C X,SUN H Z,et al.Effects of exogenous melatonin on performance,serum indexes and melatonin content of laying hens during later laying period[J].Chinese Journal of Animal Nutrition,2020,32(5):177-188.(in Chinese)
[10] YUAN J M,ROSHDY A R,GUO Y M,et al.Effect of dietary vitamin A on reproductive performance and immune response of broiler breeders[J].PLoS One,2014,9(8):e105677.
[11] FERNANDES J I M,MURAKAMI A E,SCAPINELLO C,et al.Effect of vitamin K on bone integrity and eggshell quality of white hen at the final phase of the laying cycle[J].Revista Brasileira de Zootecnia,2009,38(3):488-492.  
[12] GAN L P,FAN H,NIE W,et al.Ascorbic acid synthesis and transportation capacity in old laying hens and the effects of dietary supplementation with ascorbic acid[J].Journal of Animal Science and Biotechnology,2018,9:71.
[13] 徐昌领,左建军,冯定远.组合型木聚糖酶对21日龄黄羽肉鸡器官指数、内源消化酶活性以及肠道吸收功能的影响[J].饲料工业,2011(增刊):49-53. XU C L,ZUO J J,FENG D Y.Effect of combination xylanase on organ index,endogenous digestive enzyme activity and absorptive function of intestinal tract for yellow-feather broilers[J].Feed Industry,2011(Suppl.):49-53.(in Chinese)
[14] 王利华.不同维生素A添加量对肉鸡生产性能、免疫器官指数及血清酶活性的影响[J].中国饲料,2009(8):24-25,28. WANG L H.Effects of different vitamin A supplementation on growth performance,immune organ index and serum enzyme activity of broilers[J].China Feed,2009(8):24-25,28. (in Chinese)
[15] 董以雷,井庆川,刘雪兰,等.维生素A对罗斯308×海兰褐肉杂鸡生长性能、钙磷代谢和免疫功能的影响[J].动物营养学报,2019,31(2):629-637. DONG Y L,JIN Q C,LIU X L,et al.Effects of vitamin A on growth performance,calcium and phosphorus metabolism and immune function of Ross 308×Hyline brown hybrid broilers[J].Chinese Journal of Animal Nutrition,2019,31(2):629-637.(in Chinese)
[16] AYUSO M,FERNÁNDEZ A,ISABEL B,et al.Long term vitamin a restriction improves meat quality parameters and modifies gene expression in Iberian pigs[J].Journal of Animal Science,2015,93(6):2730-2744.  
[17] SOLOMON L W,ERDMAN J W,Jr.Vitamin A induced hypertriglyceridemia in cholesterol-fed rats[J].Lipids,1980,15(3):157-162.  
[18] YEHYA A,BAER J T,SMILEY W,et al.Hypervitaminosis A altering the lipid profile in a hypercholesterolemic patient[J].Journal of Clinical Lipidology,2009,3(3):205-207.  
[19] AHMAD S,AHSAN-UL-HAQ,YOUSAF M,et al.Production of n-3 PUFA enriched eggs by feeding various dietary ratios of n-6 to n-3 fatty acids and vitamin A levels to the laying hens in hot climate[J].The Journal of Poultry Science,2014,51(2):213-219.  
[20] MAXFIELD F R,TABAS I.Role of cholesterol and lipid organization in disease[J].Nature,2005,438(7068):612-621.  
[21] NRC.Nutrient requirements of poultry[S].9th ed.Washington,D.C.:National Academies Press,1994.
[22] BROWN J D,PLUTZKY J.Peroxisome proliferator-activated receptors as transcriptional nodal points and therapeutic targets[J].Circulation,2007,115(4):518-533.  
[23] MULLEN G E,YET L.Progress in the development of fatty acid synthase inhibitors as anticancer targets[J].Bioorganic & Medicinal Chemistry Letters,2015,25(20):4363-4369.  
[24] KNOBLOCH M,BRAUN S M G,ZURKIRCHEN L,et al.Metabolic control of adult neural stem cell activity by Fasn-dependent lipogenesis[J].Nature,2013,493(7431):226-230.  
[25] SEMENKOVICH C F.Regulation of fatty acid synthase (FAS)[J].Progress in Lipid Research,1997,36(1):43-53.  
[26] RONCARI D A K.Abnormalities of adipose cells in massive obesity[J].International Journal of Obesity,1990,14 Suppl.3:187-192.
[27] 张幸开.维生素A对肉牛肌内脂肪沉积及ACC/HSLPPARγ基因表达的影响[D].硕士学位论文.杨凌:西北农林科技大学,2005. ZHANG X K.The influence of intramuscular fat deposit and gene expression of ACC/HSL and PPARγ with the supplemetation of vitamin A in beef cattle[D].Master's Thesis.Yangling:Northwest A&F University,2005.(in Chinese)
[28] WRIGHT E M,HIRAYAMA B A,LOO D F.Active sugar transport in health and disease[J].Journal of Internal Medicine,2007,261(1):32-43.  
[29] POLIDORI D,SHA S,MUDALIRA S,et al.Canagliflozin lowers postprandial glucose and insulin by delaying intestinal glucose absorption in addition to increasing urinary glucose excretion:results of a randomized,placebo-controlled study[J].Diabetes Care,2013,36(8):2154-2161.  
[30] TOMIMATSU T,HORIE T.Increased Na+-dependent D-glucose transport in small intestine of retinyl palmitate treated rats[J].In Vivo,2001,15(1):81-86.
Outlines

/