Effects of Dietary Arachidonic Acid Content on Survival and Growth, Antioxidant Capacity, Arachidonic Acid Metabolic Enzyme Activities and Intestinal Microbial Composition of F2 Generation Chinese Sturgeon

WU Jinping; YANG Daiqin; DU Hao; LUO Jiang; XIONG Wei; LIU Yuan; WEI Qiwei; ZHU Jianqiang

doi:10.3969/j.issn.1006-267x.2021.11.052

2021 , Vol. 33 >Issue 11: 6534 - 6547

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.11.052

SHORT COMMUNICATION

Effects of Dietary Arachidonic Acid Content on Survival and Growth, Antioxidant Capacity, Arachidonic Acid Metabolic Enzyme Activities and Intestinal Microbial Composition of F₂ Generation Chinese Sturgeon

WU Jinping ,
YANG Daiqin ,
DU Hao ,
LUO Jiang ,
XIONG Wei ,
LIU Yuan ,
WEI Qiwei ,
ZHU Jianqiang

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1. College of Agriculture, Yangtze University, Jinzhou 434025, China;
2. Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China

Received date: 2021-03-30

Online published: 2021-11-10

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Abstract

The effects of arachidonic acid (ARA) on survival and growth, antioxidant capacity, tissue ARA metabolic enzyme activities and intestinal microbial composition of the F₂ generation Chinese sturgeon were evaluated in this experiment. Four isonitrogenous and isoenergetic experimental diets were formulated by adding 0, 0.5%, 1.0% and 2.0% purified ARA oil (43% ARA in total fatty acids) in the basal diet, and dietary arachidonic acid content was 0.18%, 0.53%, 0.96% and 1.89%, respectively. The F₂ generation Chinese sturgeon with an initial body weight of (4.17±0.22) kg were fed for 22 weeks with 3 replicates per diet treatment, and 18 fish per replicate. The results showed that the weight gain rate and survival rate were not significantly affected by dietary ARA content (P>0.05). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) in the liver were increased with dietary ARA content increasing, while the content of malonic aldehyde (MDA) in the liver was decreased with dietary ARA content increasing. The activities of SOD, CAT, GST and the content of MDA in the serum were not significantly affected by dietary ARA content (P>0.05). In the gonad, the activities of cyclooxygenase-2 (COX-2) and cytochrome P450 (CYP₄₅₀) of the 1.89% ARA group were significantly higher than those of the 0.18% and 0.53% ARA groups (P<0.05), but there were no significant differences between the 1.89% and 0.96% ARA groups (P>0.05); the cyclooxygenase-1 (COX-1) activity of the 0.96% ARA group was significantly higher than that of the 0.18% and 0.53% ARA groups (P<0.05); the lipoxygenase (LOX) activity of the 1.89% ARA group was significantly higher than that of the 0.18% ARA group (P<0.05). In the liver, the activities of COX-1, COX-2 and LOX were the highest in the 1.89% ARA group, and there were no significant differences between the 0.96% and 1.89% ARA groups (P>0.05); the CYP₄₅₀ activity of the 0.18% ARA group was significantly lower than that of the 0.96% and 1.89% groups (P<0.05). The Chao1 index, ACE index, Shannon index and Observed-species number of the 0.18% ARA group were significantly higher than those of the 0.53% ARA group (P<0.05), and there were no significant differences among the 0.18%, 0.96% and 1.89% ARA groups (P>0.05). The Simpson index and PD-whole tree index were not significantly affected by dietary ARA content (P>0.05). These results indicates that dietary with 1.0% ARA has no significant effects on the growth and survival of F₂ Chinese sturgeon, but can enhance the antioxidant capacity, tissue ARA metabolic enzyme activities and regulate the diversity of intestinal microbes.

Key words： arachidonic acid; Chinese sturgeon; growth; survival; antioxidant capacity; ARA metabolic enzymes; microbial diversity

Cite this article

WU Jinping , YANG Daiqin , DU Hao , LUO Jiang , XIONG Wei , LIU Yuan , WEI Qiwei , ZHU Jianqiang . Effects of Dietary Arachidonic Acid Content on Survival and Growth, Antioxidant Capacity, Arachidonic Acid Metabolic Enzyme Activities and Intestinal Microbial Composition of F₂ Generation Chinese Sturgeon[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6534 -6547 . DOI: 10.3969/j.issn.1006-267x.2021.11.052

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