POULTRY NUTRITION AND FEED

Effects of Dietary Tibetan Medicine “Dali” on Performance, Egg Quality, Serum Cytokine Content and Antioxidant Activity of Xueyu White Chickens in Laying Hens during Late Period of Laying

  • SHI Hairen ,
  • MA Xueying ,
  • WANG Yan ,
  • PENG Da ,
  • ZANG Lei ,
  • LIU Huijie ,
  • FENG Jing
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  • 1. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
    2. Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850009, China

Received date: 2021-04-25

  Online published: 2021-12-16

Abstract

The experiment was conducted to investigate the effects of Tibetan medicine "Dali" on performance, egg quality, serum cytokine content and antioxidant activity of Xueyu white chickens in laying hens during late period of laying. In a single-factor experiment design, a total of 960 laying hens (390-day-old) Xueyu white chickens with similar egg laying rate, body weight and good health were randomly divided into 6 groups with 4 replicates in each group and 40 layers in each replicate. The control group was fed a basal diet, and the experimental groups were fed the basal diet with 100, 200, 500, 800, 1 000 g/t Tibetan medicine "Dali" dry powder, respectively. The trial pre-period was 1 week, and the formal period was 8 weeks. The results showed as follows:1) in terms of performance, compared with the control group on the 4th and 8th weekend, the production rate increased significantly (P<0.01), and both the spoiled egg rate and the mortality rate were extremely significantly decreased (P<0.01). 2) In terms of egg quality, at the 8th weekend of the experiment, compared with the control group, the Haugh unit of the 800 and 1 000 g/t groups increased significantly (P<0.01). At the 4th weekend of the experiment, Haugh unit was increasing, but the difference was not significant (P>0.05). 3) In terms of antioxidant performance, compared with the control group, the test group serum glutathione peroxidase, superoxide dismutase activities and total antioxidant ability increased significantly (P<0.01), and serum malondialdehyde content decreased significantly (P<0.01). 4) In terms of cytokines, compared with the control group, the serum cytokine content had changed. Among them, the interferon-γ content of the 1 000 g/t group increased significantly (P<0.05), and the interleukin-10 content of the 1 000 g/t group increased significantly (P<0.01). In summary, the addition of Tibetan medicine "Dali" to the diet can increase the egg production rate in laying hens during late period of laying, reduce spoiled egg rate and mortality rate, increase the Haugh unit, and improve the body's antioxidant capacity. Among them, 1 000 g/t Tibetan medicine "Dali" has the best effect.

Cite this article

SHI Hairen , MA Xueying , WANG Yan , PENG Da , ZANG Lei , LIU Huijie , FENG Jing . Effects of Dietary Tibetan Medicine “Dali” on Performance, Egg Quality, Serum Cytokine Content and Antioxidant Activity of Xueyu White Chickens in Laying Hens during Late Period of Laying[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 6782 -6791 . DOI: 10.3969/j.issn.1006-267x.2021.12.020

References

[1] 闫如霞,唐德富,金贺伟,等.中草药饲料添加剂在鸡生产中的应用[J].中国饲料,2021(7):50-55. YAN R X,TANG D F,JIN H W,et al.Application of Chinese herbal medicine feed additives in chicken production[J].China Feed,2021(7):50-55.(in Chinese)
[2] 郝晨曲,马云,冯帆,等.中草药饲料添加剂主要生物学功能及应用[J].草业科学,2020,37(8):1638-1645. HAO C Q,MA Y,FENG F,et al.Primary biological function and application of Chinese herbal feed additives[J].Pratacultural Science,2020,37(8):1638-1645.(in Chinese)
[3] 黄宇,兰莎,张艺,等.HPLC测定藏药樱草杜鹃中金丝桃苷、槲皮苷和槲皮素的含量[J].中国实验方剂学杂志,2013,19(14):117-120. HUANG Y,LAN S,ZHANG Y,et al.Determination of hyperosid,quercetrin and quercetin in rhododendron primulaeflorum by HPLC[J].Chinese Journal of Experimental Traditional Medical Formulae,2013,19(14):117-120.(in Chinese)
[4] 冯冠榕.藏药"达里"抗氧化有效成分研究[D].硕士学位论文.成都:成都中医药大学,2011. FENG G R.Study on antioxidant compounds of Tibetan medicine "Dali"[D].Master's Thesis.Chengdu:Chengdu University of Traditional Chinese Medicine,2011.(in Chinese)
[5] SONG X J,GUO M Y,WANG T C,et al.Geniposide inhibited lipopolysaccharide-induced apoptosis by modulating TLR4 and apoptosis-related factors in mouse mammary glands[J].Life Sciences,2014,119(1/2):9-17.
[6] 王晓翠,武书庚,张海军,等.鸡蛋蛋清品质营养调控的研究进展[J].动物营养学报,2019,31(4):1491-1498. WANG X C,WU S G,ZHANG H J,et al.Nutritional modulation of egg albumen quality of laying hens:a review[J].Chinese Journal of Animal Nutrition,2019,31(4):1491-1498.(in Chinese)
[7] GILANI A M,KNOWLES T G,NICOL C J.Factors affecting ranging behaviour in young and adult laying hens[J].British Poultry Science,2014,55(2):127-135.  
[8] 唐日益,张鑫,王继苹,等.复方中草药添加剂对蛋鸡生产性能和蛋品质的影响[J].黑龙江畜牧兽医,2020(4):95-98. TANG R Y,ZHANG X,WANG J P,et al.Effects of compound Chinese herbal medicine additives on performance and egg quality of laying hens[J].Heilongjiang Animal Science and Veterinary Medicine,2020(4):95-98.(in Chinese)
[9] 刘青翠,彭翔,张俊平,等.杜仲叶提取物对产蛋后期蛋鸡生产性能、蛋品质、蛋黄胆固醇含量及血清抗氧化指标的影响[J].动物营养学报,2018,30(1):284-292. LIU Q C,PENG X,ZHANG J P,et al.Effects of Eucommia leaves extract on performance,egg quality,yolk cholesterol content and serum antioxidant indexes of laying hens during late period of laying[J].Chinese Journal of Animal Nutrition,2018,30(1):284-292.(in Chinese)
[10] 刘家国,张宝康,赵志辉,等.不同原则中药组方对中后期蛋鸡生产性能的影响及其机理初探[J].上海农业学报,2005,21(3):50-54. LIU J G,ZHANG B K,ZHAO Z H,et al.Effect of Chinese traditional medicine prepared according to different principles on laying performance of hens at middle-late stage and its mechanism[J].Acta Agriculturae Shanghai,2005,21(3):50-54.(in Chinese)
[11] OKADA N,SHIRATA K,NIWANO M,et al.Immunosuppressive activity of a monoterpene from Eucommia ulmoides[J].Phytochemistry,1994,37(1):281-282.  
[12] LEE M H,CHO E J,CHOI E S,et al.The effect of storage period and temperature on egg quality in commercial eggs[J].Korean Journal of Poultry Science,2016,43(1):31-38.  
[13] 陈杨超,张国祖,吕明其,等.6味中药超微粉对产蛋后期蛋鸡生产性能、蛋品质和生殖激素水平影响的研究[J].黑龙江畜牧兽医,2018(8):163-165. CHEN Y C,ZHANG G Z,LV M Q,et al.The effect of ultrafine powder of six Chinese herbal medicines on the performance,egg quality and reproductive hormone levels of laying hens in the late laying stage[J].Heilongjiang Animal Science and Veterinary Medicine,2018(8):163-165.(in Chinese)
[14] 孙娜.照山白和耳叶杜鹃叶中的化学成分及其生物活性研究[D].博士学位论文.武汉:华中科技大学,2019. SUN N.Studies on the chemical constituents and bioactivities from the leaves of Rhododendron micranthum and Rhododendron auriculatum (Ericaceae)[D].Ph.D.Thesis.Wuhan:Huazhong University of Science and Technology,2019.(in Chinese)
[15] DA ROSA C E,IURMAN M G,ABREU P C,et al.Antioxidant mechanisms of the Nereidid Laeonereis acuta (Anelida:Polychaeta) to cope with environmental hydrogen peroxide[J].Physiological and Biochemical Zoology,2005,78(4):641-649.  
[16] LIU B,XIONG Y L L,JIANG J,et al.Cellular antioxidant mechanism of selenium-enriched yeast diets in the protection of meat quality of heat-stressed hens[J].Food Bioscience,2021,39:100798.
[17] 马春阳,李文强,王丽,等.复方中草药制剂对蛋鸡产蛋性能和抗氧化性能的影响[J].中国家禽,2016,38(4):58-60. MA C Y,LI W Q,WANG L,et al.Compound Chinese herbal preparations on laying performance and performance of laying hens Influence of antioxidant properties[J].China poultry,2016,38(4):58-60.(in Chinese)
[18] 陈静,朴钟云,刘显军.中草药饲料添加剂对早期断奶仔猪血清抗氧化指标的影响[J].沈阳农业大学学报,2010,41(4):477-480. CHEN J,PIAO Z Y,LIU X J.Effects of Chinese herbal additives on serum antioxidant traits of ablactation piglets[J].Journal of Shenyang Agricultural University,2010,41(4):477-480.(in Chinese)
[19] HARRINGTON L E,HATTON R D,MANGAN P R,et al.Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages[J].Nature Immunology,2005,6(11):1123-1132.  
[20] MOORE K W,DE WAAL MALEFYT R,COFFMAN R L,et al.Interleukin-10 and the interleukin-10 receptor[J].Annual Review of Immunology,2001,19:683-765.
[21] GHAYUR T,BANERJEE S,HUGUNIN M,et al.Caspase-1 processes IFN-γ-inducing factor and regulates LPS-induced IFN-γ production[J].Nature,1997,386(6625):619-623.  
[22] 耿卫朴,徐曼,罗祎,等.灵芝多糖和当归多糖促进人外周血T淋巴细胞增殖和分泌IFN-γ[J].中国药理学通报,2012,28(5):655-658. GENG W P,XU M,LUO Y,et al.The promotional effect of Ganoderma lucidum polysaccharide and Angelica sinensis polysaccharide on the proliferation and IFN secretion of human peripheral blood T lymphocytes[J].Chinese Pharmacological Bulletin,2012,28(5):655-658.(in Chinese)
[23] 邱妍,崔保安,胡元亮,等.4种多糖对鸡淋巴细胞中IL-4、IFN-γ mRNA表达的影响[J].江苏农业科学,2009(2):32-35. QIU Y,CUI B A,HU Y L,et al.Effects of four polysaccharides on mRNA expression of IL-4、IFN-γ of lymphocyte in chicken[J].Jiangsu Agricultural Sciences,2009(2):32-35.(in Chinese)
[24] SARA A M,BARRI M E,SALWAR M E,et al.The implications of sunflower seeds and flax seeds on plasma lipids profile and fatty acids profile-IL-6 correlation in laying hens:a comparative study[J].Scientific Research Journal,2016,4(3):1-8.
[25] BAI S P,HUANG Y,LUO Y H,et al.Alteration in lymphocytes responses,cytokine and chemokine profiles in laying hens infected with Salmonella typhimurium[J].Veterinary Immunology and Immunopathology,2014,160(3/4):235-243.
[26] TANG L P,LI W H,LIU Y L,et al.Heat stress inhibits expression of the cytokines,and NF-κB-NLRP3 signaling pathway in broiler chickens infected with Salmonella typhimurium[J].Journal of Thermal Biology,2021,98:102945.
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