RESEARCH PAPER

Effects of Polyunsaturated Fatty Acid Combination on Meat Quality, Serum Antioxidant Indexes and Conjugated Linoleic Acid Content in Longissimus dorsi of Tan Sheep

  • MA Xiuhua ,
  • HU Zhiqiang ,
  • QI Mingjiang ,
  • ZHOU Yuxiang ,
  • YANG Wanzong
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  • 1. College of Agriculture, Ningxia University, Yinchuan 750021, China;
    2. Pengyang County Animal Husbandry Technology Extension Service Center, Pengyang 756500, China

Received date: 2021-05-27

  Online published: 2022-01-18

Abstract

In this experiment, in order to explore the effects of the combination of polyunsaturated fatty acids on the meat quality, serum antioxidant indexes and conjugated linoleic acid content in longissimus dorsi of Tan sheep, 27 Tan sheep male lambs at 4 months of age were randomly divided into three groups as the control group (basic diet), test group Ⅰ (basic diet+1% soybean oil+1% schizochytrium powder), and test group Ⅱ (basic diet+1% linseed oil+1% schizochytrium powder) were measured for daily weight gain, meat quality, serum antioxidant indexes and conjugated linoleic acid content of each group. The pre-experiment lasted for 15 days and the experimental period lasted for 60 days. The results showed that compared with control group, the average daily weight gain of Tan sheep in the diet with the fat-rich polyunsaturated fatty acid (PUFA) combination had no significant effect (P>0.05); the meat shear force of the test group Ⅰ and test group Ⅱ was significantly lower than that of the control group(P<0.05), and the redness (a*) value was significantly greater than that of the control group (P<0.05), and the lightness (L*) value of the test group Ⅱ was significantly lower than that of the test group Ⅰ and the control group (P<0.05). In addition, the marbling scores of the two test groups was higher than that of the control group. The content of trans-11 vaccenic acid in longissimus dorsi in test group Ⅱ was higher than that of the other two groups, and the content of conjugated linoleic acid (CLA) in test group Ⅰ and test group Ⅱ was significantly higher than that of the others two groups (P<0.05). The contents of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in longissimus dorsi in the two test groups also increased; n-3 polyunsaturated fatty acids (n-3PUFA) content among three groups had no significant effect (P>0.05). The content of C18:3n-3 in longissimus dorsi in test group Ⅰ was higher than that of other groups, and the content of C20:3n-6 of test group Ⅱ was higher. To sum up, adding soybean oil+schizochytrium powder, linseed oil+schizochytrium powder to the diet can improve Tan sheep tenderness and improve flesh color, and can also make the intramuscular fat distribution more even and help increase the content of intramuscular CLA without affecting the growth performance of Tan sheep and the antioxidant capacity of serum.

Cite this article

MA Xiuhua , HU Zhiqiang , QI Mingjiang , ZHOU Yuxiang , YANG Wanzong . Effects of Polyunsaturated Fatty Acid Combination on Meat Quality, Serum Antioxidant Indexes and Conjugated Linoleic Acid Content in Longissimus dorsi of Tan Sheep[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 457 -466 . DOI: 10.3969/j.issn.1006-267x.2022.01.043

References

[1] COHUET G,STRUIJKER-BOUDIER H.Mechanisms of target organ damage caused by hypertension:therapeutic potential[J].Pharmacology & Therapeutics,2006,111(1):81-98.  
[2] 乔立君,郑征,马文慧,等.多不饱和脂肪酸对大鼠肠道菌群及脂肪代谢相关基因的影响[J].食品科学,2014,35(17):231-235. QIAO L J,ZHENG Z,MA W H,et al.Effect of PUFA-containing diet on gut microbiota and fat metabolism-related genes in rats[J].Food Science,2014,35(17):231-235.(in Chinese)
[3] VILADOMIU M,HONTECILLAS R,BASSAGANYA-RIERA J.Modulation of inflammation and immunity by dietary conjugated linoleic acid[J].European Journal of Pharmacology,2016,785:87-95.
[4] LEE J H,KIM B,HWANG C E,et al.Changes in conjugated linoleic acid and isoflavone contents from fermented soymilks using Lactobacillus plantarum P1201 and screening for their digestive enzyme inhibition and antioxidant properties[J].Journal of Functional Foods,2018,43:17-28.
[5] EDWARDS H D,SHELVER W L,CHOI S,et al.Immunogenic inhibition of prominent ruminal bacteria as a means to reduce lipolysis and biohydrogenation activity in vitro[J].Food Chemistry,2017,218:372-377.
[6] BAKER E J,MILES E A,BURDGE G C,et al.Metabolism and functional effects of plant-derived omega-3 fatty acids in humans[J].Progress in Lipid Research,2016,64:30-56.
[7] DÁVILA-RAMÍREZ J L,AVENDAÑO-REYES L,MACÍAS-CRUZ U,et al.Fatty acid composition and physicochemical and sensory characteristics of meat from ewe lambs supplemented with zilpaterol hydrochloride and soybean oil[J].Animal Production Science,2017,57(4):767-777.  
[8] FRANCISCO A,DENTINHO M T,ALVES S P,et al.Growth performance, carcass and meat quality of lambs supplemented with increasing levels of a tanniferous bush (Cistus ladanifer L.) and vegetable oils[J].Meat Science,2015,100:275-282.
[9] FLAKEMORE A R,MALAU-ADULI B S,NICHOLS P D,et al.Omega-3 fatty acids,nutrient retention values,and sensory meat eating quality in cooked and raw Australian lamb[J].Meat Science,2017,123:79-87.
[10] 李海庆.日粮不饱和脂肪酸对滩羊体脂CLA调控作用及机理研究[D].博士学位论文.银川:宁夏大学,2019. LI H Q.Study on effects and mechanism of dietary unsaturated fatty acids on tissue conjugated linoleic acid (CLA) synthesis of Tan sheep[D].Ph.D.Thesis.Yinchuan:Ningxia University,2019.(in Chinese)
[11] ALDAI N,DUGAN M E R,JUÁREZ M,et al.Double-muscling character influences the trans-18:1 and conjugated linoleic acid profiles in concentrate-fed yearling bulls[J].Meat Science,2010,85(1):59-65.  
[12] FAN Y X,REN C F,MENG F X,et al.Effects of algae supplementation in high-energy dietary on fatty acid composition and the expression of genes involved in lipid metabolism in Hu sheep managed under intensive finishing system[J].Meat Science,2019,157:107872.
[13] CHIKWANHA O C,VAHMANI P,MUCHENJE V,et al.Nutritional enhancement of sheep meat fatty acid profile for human health and wellbeing[J].Food Research International,2018,104:25-38.
[14] 杜迎雪,刘振国,张卫星,等.饲粮添加油菜蜂花粉对芦花鸡生产性能、蛋品质和血清生化指标的影响[J].动物营养学报,2019,31(6):2915-2926. DU Y X,LIU Z G,ZHANG W X,et al.Effects of adding rape bee pollen to diet on performance,egg quality and serum biochemical indexes of reed chicken[J].Chinese Journal of Animal Nutrition,2019,31(6):2915-2926.(in Chinese)
[15] 侯鹏霞.滩羊羔羊早期补饲以及不同体重阶段羊肉品质的研究[D].硕士学位论文.银川:宁夏大学,2014. HOU P X.Studies on the early supplementary of lamb and mutton quality of different stages of weight in Tan sheep[D].Master's Thesis.Yinchuan:Ningxia University,2014.(in Chinese)
[16] 国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品中脂肪酸的测定:GB 5009.168-2016[S].北京:中国标准出版社, 2016. National Health and Family Planning Commission of the People's Republic of China,General Administration of National Food and Drug.Determination of fatty acids in food:GB 5009.168-2016[S].Beijing:Standards Press of China,2016.(in Chinese).
[17] URRUTIA O,MENDIZABAL J A,INSAUSTI K,et al.Effects of addition of linseed and marine algae to the diet on adipose tissue development,fatty acid profile,lipogenic gene expression, and meat quality in lambs[J].PLoS One,2016,11(6):e0156765.
[18] 王雪.亚麻油与棕榈油的混合添加及亚麻籽对绒山羊体组织n-3长链多不饱和脂肪酸生成的影响及机理[D].博士学位论文.呼和浩特:内蒙古农业大学,2019. WANG X.The effects and mechanism of dietary supplementation with a combination of linseed and palm oils or linseed grain on the synthesis of n-3 long-chain polyunsaturated fatty acids in body tissues of cashemere goats[D].Ph.D.Thesis.Hohhot:Inner Mongolia Agricultural University,2019.(in Chinese)
[19] 张秋旭.不同形式亚麻油对肉羊生产性能、肌肉脂肪酸含量和脂肪代谢的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2018. ZHANG Q X.Effects of different forms of linseed oil on growth performance,fatty acid content and lipid metabolism in muscle of sheep[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2018.(in Chinese)
[20] PALMQUIST D L.Digestibility of cotton lint fiber and whole oilseeds by ruminal microorganisms[J].Animal Feed Science and Technology,1995,56(3/4):231-242.
[21] 王飞扬,田川尧,蔡锋隆,等.不同种类油脂对雏鸡机体ω-6/ω-3值、生长性能、内脏指数、养分表观代谢率及血清生化指标的影响[J].动物营养学报,2021,33(2):841-849. WANG F Y,TIAN C Y,CAI F L,et al.Effects of different kinds of oil on body ω-6/ω-3 value,growth performance,visceral indexes,nutrient apparent metabolic rates and serum biochemical indexes of chickens[J].Chinese Journal of Animal Nutrition,2021,33(2):841-849.(in Chinese)
[22] 王雪.复合化学处理稻草饲粮中添加甘露寡糖对滩羊生产性能及肉品质的影响[D].硕士学位论文.银川:宁夏大学,2020. WANG X.Effects of mannan oligosaccharides added in compound chemical treatment straw diet on production performance and meat quality of Tan sheep[D].Master's Thesis.Yinchuan:Ningxia University,2020.(in Chinese)
[23] 温琦.放牧补饲条件下绒山羊羯羔与母羔育肥性能、屠宰性能及肉品质的比较研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2018. WEN Q.Study on comparison of fattening performance and slaughter performance and meat quality between cashmere castrated lambs and ewe lambs under the condition of grazing supplementary feeding[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2018.(in Chinese)
[24] 赵天章.日粮油脂类型对羊肉脂肪酸和肌内脂肪含量的影响及其机理[D].博士学位论文.北京:中国农业大学,2014. ZHAO T Z.Effect and the initial mechanism research of dietary oil sources on fatty acid and intramucular fat contents of lamb[D].Ph.D.Thesis.Beijing:China Agricultural University,2014.(in Chinese)
[25] 孙德文.糖萜素对鸡肉品质的影响及其作用机理研究[D].硕士学位论文.杭州:浙江大学,2003. SUN D W.Effect of saccharicterpenin on broilers meat quality and approach to its mechanism[D].Master's Thesis.Hangzhou:Zhejiang University,2003.(in Chinese)
[26] 王敏.放牧与舍饲对肉牛生产性能和肉品质影响的比较研究[D].博士学位论文.长春:吉林大学,2020. WANG M.A comparative study on the effects of grazing and stall feeding on the performance and meat quality of beef cattle[D].Ph.D.Thesis.Changchun:Jilin University,2020.(in Chinese)
[27] DÍAZ M T,PÉREZ C,SÁNCHEZ C I,et al.Feeding microalgae increases omega-3 fatty acids of fat deposits and muscles in light lambs[J].Journal of Food Composition and Analysis,2017,56:115-123.
[28] PONNAMPALAM E N,BURNETT V F,NORNG S,et al.Muscle antioxidant (vitamin E) and major fatty acid groups,lipid oxidation and retail colour of meat from lambs fed a roughage based diet with flaxseed or algae[J].Meat Science,2016,111:154-160.
[29] 殷雨洋,任韵,黄杰,等.青贮饲用油菜对湖羊血液生化指标和抗氧化能力的影响[J].上海畜牧兽医通讯,2021(1):21-23. YIN Y Y,REN Y,HUANG J,et al.Effects of rape silage on blood biochemical indexes and antioxidant ability of Hu sheep[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine,2021(1):21-23.(in Chinese)
[30] SALTER A M.Dietary fatty acids and cardiovascular disease[J].Animal,2013,7(1):163-171.
[31] 刘仕军.二十二碳六烯酸和亚油酸对牛体内共轭亚油酸前体物累积的影响[D].博士学位论文.北京:中国农业科学院,2008. LIU S J.Effect of docosahexaenoic acid and linoleic acid on accumulation of trans-vaccenic acid in cattle[D].Ph.D.Thesis.Beijing:The Chinese Academy of Agricultural Sciences,2008.(in Chinese)
[32] 于淇.日粮添加不同植物油对猪生长性能、肉质及健康的影响[D].硕士学位论文.兰州:西北民族大学,2020. YU Q.Effects of dietary different vegetable oils on growth performance,meat quality and health of pigs[D].Master's Thesis.Lanzhou:Northwest University for Nationalities,2020.(in Chinese)
[33] 张东伟.瘤胃发酵及脂肪酸组成的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2019. ZHANG D W.Effects of different ratios of linseed oil and palm oil on rumen fermentation and fatty acid composition of cashmere goats[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2019.(in Chinese)
[34] VALENZUELA R,ECHEVERRIA F,ORTIZ M,et al.Hydroxytyrosol prevents reduction in liver activity of Δ-5 and Δ-6 desaturases,oxidative stress, and depletion in long chain polyunsaturated fatty acid content in different tissues of high-fat diet fed mice[J].Lipids in Health and Disease,2017,16(1):64.
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