Evaluation of Fermentation Quality of Corn Straw Silage Treated with Different Additives Based on Principal Component Analysis and Membership Function Analysis

ZHANG Zhiheng; WANG Yuqin; REN Guoyan; LI Yuanxiao; ZHAO Lingping; WU Qiujue; ZHANG Zijun

doi:10.3969/j.issn.1006-267x.2022.04.061

2022 , Vol. 34 >Issue 4: 2677 - 2688

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.061

Evaluation of Fermentation Quality of Corn Straw Silage Treated with Different Additives Based on Principal Component Analysis and Membership Function Analysis

ZHANG Zhiheng ,
WANG Yuqin ,
REN Guoyan ,
LI Yuanxiao ,
ZHAO Lingping ,
WU Qiujue ,
ZHANG Zijun

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1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
2. Engineering Research Center for Mutton Sheep Breeding of Henan Province, Luoyang 471003, China;
3. Anhui Agricultural University, Hefei 230000, China

Received date: 2021-08-29

Online published: 2022-04-14

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Abstract

The purpose of this experiment was to study the effects of different additives on conventional nutrient contents and fermentation quality of corn straw silage. The 'Yuqing 386' corn straw after seed harvest was used as the experimental material, two additives of antimicrobial peptide-producing Bacillus subtilis (BS) and cellulase (CL) were added to the crushed corn straw at 3 different doses of 0.01%, 0.02% and 0.03%, respectively, and no additive was used as the blank control group (CK group). A total of 7 groups with 3 replicates per group were stored in the dark at room temperature for 110 days, and the conventional nutrient contents, lignocellulosic components and fermentation quality were determined and analyzed. The results showed as follows:1) compared with the CK group, the content of water-soluble carbohydrate in 0.01% CL group, 0.02% CL group and 0.01% BS group decreased significantly (P<0.05), and the content of dry matter in 0.03% CL group increased significantly (P<0.05); the content of crude protein in 0.01% BS group was significantly higher than that in 0.03% BS group (P<0.05). 2) Compared with the CK group, the contents of neutral detergent fiber, acid detergent fiber, cellulose and holocellulose in 0.01% CL group decreased significantly (P<0.05), the contents of neutral detergent fiber, acid detergent fiber and acid detergent lignin in 0.02% CL group decreased significantly (P<0.05), and the relative feeding value in 0.01% CL group and 0.02% CL group increased significantly (P<0.05). 3) Compared with the CK group, the content of lactic acid in all BS added groups and 0.02% CL group increased significantly (P<0.05); there was no significant difference in pH between each additive group and CK group (P>0.05), but the pH in 0.03% BS group was significantly lower than that in 0.01% BS group and 0.01% CL group (P<0.05); there was no significant difference in ammoniacal nitrogen/total nitrogen among all groups (P>0.05). 4) Principal component analysis and membership function analysis showed that the comprehensive ranking of each group was 0.02% CL group>0.03% CL group>0.01% BS group>0.03% BS group>0.01% CL group>0.02% BS group>CK group. In conclusion, both CL and BS can improve the conventional nutrient contents and fermentation quality of corn straw silage at a certain extent, and the optimum doses of CL and BS are 0.02% and 0.01%, respectively. Among all additive groups, 0.02% CL group has the best silage effect.

Key words： corn stalk; fermentation quality; silage additives; principal component analysis; membership function evaluation

Cite this article

ZHANG Zhiheng , WANG Yuqin , REN Guoyan , LI Yuanxiao , ZHAO Lingping , WU Qiujue , ZHANG Zijun . Evaluation of Fermentation Quality of Corn Straw Silage Treated with Different Additives Based on Principal Component Analysis and Membership Function Analysis[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2677 -2688 . DOI: 10.3969/j.issn.1006-267x.2022.04.061

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