RESEARCH PAPER

Effects of Non-Fiber Carbohydrates/Neutral Detergent Fiber Value and Mixed Probiotics Supplemental Level on Fermented Quality and Mycotoxin Contents of Fermented Total Mixed Ration with Broussonetia papyrifera

  • CHEN Guangji ,
  • XIONG Xianqin ,
  • HE Runxia ,
  • TIAN Xiong ,
  • ZOU Xiaomin ,
  • YANG Hong ,
  • ZHANG Kun ,
  • SHANG Yishun ,
  • LI Xiaodong ,
  • LI Shige ,
  • ZHANG Rong
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  • 1. Institute of Prataculture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
    2. Guizhou Sunshine Grass Technology Co., Ltd., Dushan 558200, China;
    3. Guizhou Dingxin Agriculture and Animal Husbandry Technology Co., Ltd., Guiyang 550008, China;
    4. Daozhen Gelao and Miao Autonomous County Animal Husbandry and Fisheries Service Center, Daozhen 563500, China;
    5. Wuchuan Gelao and Miao Autonomous County Animal Husbandry and Fisheries Service Center, Wuchuan 564399, China

Received date: 2021-10-30

  Online published: 2022-05-14

Abstract

This study aimed to explore the effects of non-fiber carbohydrates/neutral detergent fiber (NFC/NDF) value and mixed probiotics supplemental level on fermentation quality and mycotoxin content of fermented total mixed ration (FTMR) with Broussonetia papyrifera as the main raw material. A 2-factor cross grouping experimental design was adopted, and the two factors were NFC/NDF value (1.08, 1.34, 1.60 and 1.86) of fermentation substrate and mixed probiotics supplemental level (0, 2, 4 and 8 g/kg DM). The pH, organic acid and mycotoxin contents of each FTMR were measured after fermentation. The results showed as follows:1) the pH of fermentation substrate decreased linearly and quadratically with the increase of NFC/NDF value (P<0.05), and minimum emerged when NFC/NDF value=1.60; the organic acid contents increased quadratically with the increase of NFC/NDF value (P<0.05), and the lactic acid, acetic acid and propionic acid contents were the highest when NFC/NDF value=1.60, while the butyric acid content was the lowest when NFC/NDF value=1.60. Compared with no-added group, supplementation of 2, 4 and 8 g/kg DM mixed probiotics significantly decreased the pH (P<0.05), and significantly increased the lactic acid content (P<0.05); supplementation of 4 and 8 g/kg DM mixed probiotics significantly increased the propionic acid content (P<0.05), and significantly increased the butyric acid content (P<0.05). NFC/NDF value and mixed probiotics supplemental level had significant interaction effects on pH and organic acid contents (P<0.05). 2) NFC/NDF value had significant effects on the aflatoxin B1, deoxynivalenol and zearalenone contents in fermentation substrate (P<0.05). Three mycotoxin contents were higher when NFC/NDF value=1.08. The aflatoxin B1 content showed a significant linear and quadratic decreasing with the increase of NFC/NDF value (P<0.05), and the lowest value was found when NFC/NDF value=1.60. Mixed probiotics supplemental level had significant effects on the aflatoxin B1 and zearalenone contents in fermentation substrate (P<0.05). The aflatoxin B1 and zearalenone contents in 4 and 8 g/kg DM mixed probiotics groups were significantly lower than those in no-added group (P<0.05). NFC/NDF value and mixed probiotics supplemental level had significant interaction effects on aflatoxin B1 and zearalenone contents (P<0.05). 3) The pH of fermentation substrate had an extremely significant positive correlation with the three mycotoxin contents (P<0.01), while the lactic acid content had an extremely significant negative correlation with them (P<0.01), then the acetic acid and propionic acid contents had an extremely significant or a significant negative correlation with aflatoxin B1 content (P<0.05), and the butyric acid content had an extremely significant positive correlation with the aflatoxin B1 and zearalenone contents (P<0.01). Therefore, it is found that the NFC/NDF value, mixed probiotics supplemental level and the interaction of them had significant effects on the fermentation quality and mycotoxin contents of FTMR with Broussonetia papyrifera. The appropriate NFC/NDF value is 1.60 and the appropriate mixed probiotics supplemental level is 4 g/kg DM. There is a negative correlation between the lactic acid, acetic acid and propionic acid contents and the mycotoxin contents of fermentation substrate.

Cite this article

CHEN Guangji , XIONG Xianqin , HE Runxia , TIAN Xiong , ZOU Xiaomin , YANG Hong , ZHANG Kun , SHANG Yishun , LI Xiaodong , LI Shige , ZHANG Rong . Effects of Non-Fiber Carbohydrates/Neutral Detergent Fiber Value and Mixed Probiotics Supplemental Level on Fermented Quality and Mycotoxin Contents of Fermented Total Mixed Ration with Broussonetia papyrifera[J]. Chinese Journal of Animal Nutrition, 2022 , 34(5) : 3163 -3173 . DOI: 10.3969/j.issn.1006-267x.2022.05.043

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