RESEARCH PAPER

Effects of Adding Clostridium butyricum in Diets on Growth Performance, Serum Biochemical Indices, Immune Function and Antioxidant Ability of Yellow Catfish (Pelteobagrus fulvidraco)

  • WANG Hairui ,
  • MO Wenyan ,
  • ZHAO Hongxia ,
  • HUANG Wen ,
  • ZHENG Chuntian ,
  • CAO Junming
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  • Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2022-01-20

  Online published: 2022-08-11

Abstract

This experiment was aimed to study the effects of adding Clostridium butyricum in diets on growth performance, serum biochemical indices, immune function and antioxidant ability of yellow catfish (Pelteobagrus fulvidraco). A total of 450 yellow catfish with an initial body weight of (5.55±0.05) g were randomly divided into 5 groups with 3 replicates per group and 30 fish per replicate. Five experimental diets were prepared by adding 0, 2, 20, 200 and 2 000 mg/kg Clostridium butyricum into the basal diet, named as G0, G1, G2, G3 and G4, respectively. The number of Clostridium butyricum in the diets was 0, 4.8×106, 4.5×107, 5.1×108 and 3.6×109 CFU/kg, respectively. The feeding period was 56 days. Results showed as follows: 1) compared with G0 group, the weight gain rate of yellow catfish in G1, G2 and G3 groups was significantly increased (P<0.05), but the feed conversion ratio was significantly decreased (P<0.05). 2) The serum glutamic-pyruvic transaminase activity in G3 and G4 groups was significantly lower than that in G0 group (P<0.05), and the serum glutamic-pyruvic transaminase activity in G3 group was significantly lower than that in G0 group (P<0.05). Serum glucose content of yellow catfish in G1, G2 and G3 groups was significantly higher than that in G0 group (P<0.05). 3) Compared with G0 group, the serum glutathione, lysozyme and superoxide dismutase activities in G3 group were significantly increased (P<0.05), the serum complement 3 content and alkaline phosphatase activity in G4 group were significantly increased (P<0.05), and the serum immunoglobulin M content in G3 and G4 groups was significantly increased (P<0.05). The serum total antioxidant capacity of yellow catfish in Clostridium butyricum group was significantly higher than that in G0 group (P<0.05). 4) Compared with G0 group, intestinal short-chain fatty acid content in G2, G3 and G4 groups was significantly increased (P<0.05). In conclusion, it is recommended that the supplemental level of Clostridium butyricum is 200 mg/kg (5.1×108 CFU/kg) based on growth performance, serum immune and antioxidant indexes as evaluation criteria.

Cite this article

WANG Hairui , MO Wenyan , ZHAO Hongxia , HUANG Wen , ZHENG Chuntian , CAO Junming . Effects of Adding Clostridium butyricum in Diets on Growth Performance, Serum Biochemical Indices, Immune Function and Antioxidant Ability of Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5295 -5303 . DOI: 10.3969/j.issn.1006-267x.2022.08.050

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