RESEARCH PAPER

Optimization of Exogenous Complex Non-Starch Polysaccharidases Ratio and Their Effect on in Vitro Fermentation Characteristics

  • XUE Yuyang ,
  • SUN Haobin ,
  • WANG Yinling ,
  • LI Jiacheng ,
  • LIU Baocang ,
  • HAN Kun ,
  • QI Yayin ,
  • CHEN Cheng ,
  • ZHANG Wenju
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  • 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
    2. Xinjiang Taikun Group Changji Feed Limited Liability Company, Changji 831199, China

Received date: 2022-04-28

  Online published: 2022-11-14

Abstract

The aim of this experiment was to investigate the optimal ratio of exogenous complex non-amylose polysaccharidases and their effect on in vitro fermentation characteristics. A single-factor completely randomized experimental design was used in this experiment, in which four single enzymes (cellulase, xylanase, β-glucanase and mannanase) were added to the diet of weaned fattening lambs at seven levels, with three replicates for each level. The optimal addition of the single enzymes was determined using gas production (GP) and dry matter degradation rate (DMD) as evaluation indexes. Based on the above results, the four enzymes were combined in a compound combination using an orthogonal design 4 factors and 3 levels L9 (34) test to determine the optimal ratio. The in vitro fermentation was carried out by adding the exogenous complex non-amylose polysaccharidases with optimal ratio to a basal diet (test group) compared with the basal diet (control group), and the GP, DMD and fermentation parameters were determined at different fermentation time (2, 4, 6, 8, 12, 24, 48 h). The results showed that the optimal addition of the four single enzymes used the diet of weaned fattening lambs as substrate were 750 U/g for cellulase, 125 U/g for xylanase, 750 U/g for β-glucanase and 125 U/g for mannanase, and the optimal ratio of GP and DMD for the reaction catalyzed by this complex enzymes were cellulase:xylanase:β-glucanase:mannanase=1 000:250:500:50 (U/g, dry matter basis). The application results of the exogenous complex non-amylose polysaccharidases with optimal ratio in the rumen in vitro fermentation showed that, after 48 h of incubation, the pH of the fermentation broth was at a normal level in all groups; the average GP, DMD and NH3-N concentration of the test group was extremely significantly higher than those of the control group (P<0.05); the microbial protein (MCP) concentration of the test group was lower than that of the control group, but the difference was not significant (P>0.05). The differences in propionic acid, butyric acid, total volatile fatty acid (TVFA) concentrations and acetic acid/propionic acid (A/P) between the test group and the control group were extremely significant (P<0.01). In conclusion, the addition of exogenous complex non-amylose polysaccharidases with optimal ratio[cellulase:xylanase:β-glucanase:mannanase=1 000:250:500:50 (U/g, dry matter basis)] can increase rumen in vitro fermentation GP, DMD, and improve the rumen fermentation function, which has the potential as accelerating the digestion of dietary nutrients.

Cite this article

XUE Yuyang , SUN Haobin , WANG Yinling , LI Jiacheng , LIU Baocang , HAN Kun , QI Yayin , CHEN Cheng , ZHANG Wenju . Optimization of Exogenous Complex Non-Starch Polysaccharidases Ratio and Their Effect on in Vitro Fermentation Characteristics[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7431 -7442 . DOI: 10.3969/j.issn.1006-267x.2022.11.060

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